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FLIM Systems

 

   FLIM Systems for Zeiss LSM 710

Multiphoton NDD FLIM

Confocal FLIM

Single-detector systems

Dual-detector systems

Multi-spectral FLIM systems

Simultaneous detection in all wavelength channels

Parallel TCSPC systems for dual-detector systems

Unprecedented count rate

Short acquisition time for bright samples

Sequential recording mode by memory swapping

FLIM sequences as fast as 2 images / second

Time-tag and histogram modes

Extremely large images by FIFO imaging mode
 

Data sheet, Handbook

 

  DCS-120 Confocal FLIM Scanning Systems

Complete laser scanning FLIM microscopes

FLIM upgrade for conventional microscopes

One or two bh BDL-SMC picosecond diode lasers

Laser wavelengths 405 nm, 440 nm, 473, or 640 nm

Scanning by fast galvano mirrors

Frame times down to <100 ms, line times down to 1 ms

Frame scan, line scan, and point (FCS) capability

Two fully confocal detection channels

Individually selectable pinholes

Individually selectable filters

Secondary beamsplitter wheel with dichroic and polarising beamsplitters

High efficiency due to direct-coupled detectors

PMC-100, H7422, ultra-fast MCP PMT, or SPAD detector modules

Multi-spectral FLIM detector module

Fully integrated scanning, detector and laser control

Single-channel and dual-channel (parallel detection) TCSPC systems

On-board image acquisition and time-tag imaging modes

Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems

Instrument and data analysis software for Windows 2000, NT, XP, or Vista

 

 

Data sheet, Handbook

 

 FLIM Systems for Zeiss LSM 510 NLO Microscopes
(Multiphoton)

Non-descanned detection

High efficiency, deep-tissue imaging capability

Single-detector systems

Dual-detector systems

Multi-spectral FLIM systems

PMC-100, H7422, ultra-fast MCP PMT, multispectral PMT modules

Detectors protected by shutters and by overload shutdown

Single-channel and dual-channel (parallel detection) TCSPC systems

On-board image acquisition and time-tag imaging modes

Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems

Instrument and data analysis software for Windows 2000, NT, XP, or Vista

Upgrades for all LSM 510 NLO versions, upright or inverted microscopes

Zeiss NDD switch box must be installed
 

Data sheet, Handbook, Multispectral detector handbook

 

 FLIM Systems for Zeiss LSM 510 Microscopes
 (One-Photon)

Confocal detection

Excitation by bh BDL-405-SMC picosecond diode laser

Single-detector systems

Dual-detector systems

Multi-spectral FLIM systems

PMC-100, H7422, ultra-fast MCP PMT, multispectral PMT, and SPAD detector modules

Detectors protected by overload shutdown

Single-channel and dual-channel (parallel detection) TCSPC systems

On-board image acquisition and time-tag imaging modes

Electronics integrated in bh Simple-Tau 150, 152, 830 or standard-PC systems

Instrument and data analysis software for Windows 2000, NT, XP, or Vista

Upgrades for all LSM 510 confocal versions, upright or inverted microscopes

Requirements:
Fibre output from LSM 510 scan head must be installed
Input fibre for 405 nm laser must be availableNon-descanned detection

 




Data sheet, Handbook, Multispectral detector handbook

 

Non-Descanned FLIM Systems for Leica SP2 and SP5 ( Multiphoton )

Non-descanned detection systems

Detection via RLD port of microscope

High efficiency, deep-tissue imaging capability

Single-detector systems and dual-detector systems

Multi-spectral FLIM systems

PMC-100, H7422, ultra-fast MCP PMT, multispectral PMT modules

Detectors protected by shutter and by overload shutdown

Single-channel and dual-channel (parallel detection) TCSPC systems

On-board image acquisition and time-tag imaging modes

Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems

Instrument and data analysis software for Windows 2000, NT, XP, or Vista

Requirement: RLD port must be installed at SP2 or SP5 microscope
 

Data sheet, Handbook, Multispectral detector handbook

 

Descanned FLIM Systems for Leica SP2 and SP5 Multiphoton Microscopes

Descanned detection systems

Detection via X1 port of microscope

High efficiency for thin samples

High suppression of background light

Single-detector systems and dual-detector systems

Multi-spectral FLIM systems

PMC-100, H7422, ultra-fast MCP PMT, multispectral PMT modules

Detectors protected by shutter and by overload shutdown

Single-channel and dual-channel (parallel detection) TCSPC systems

On-board image acquisition and time-tag imaging modes

Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems

Instrument and data analysis software for Windows 2000, NT, XP, or Vista

Handbook, Multispectral detector handbook

Confocal FLIM Systems for Leica SP2 Microscopes

Descanned confocal detection systems

Detection via X1 port of microscope

Single-detector systems

Multi-spectral FLIM systems

PMC-100, H7422, ultra-fast MCP PMT, multispectral PMT modules

Detectors protected by shutter and by overload shutdown

Single-channel and dual-channel (parallel detection) TCSPC systems

On-board image acquisition and time-tag imaging modes

Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems

Instrument and data analysis software for Windows 2000, NT, XP, or Vista

Requirement: X1 port adaptation must be installed at SP2 or SP5 microscope

Handbook, Multispectral detector handbook

Confocal FLIM Systems for Leica SP5 Microscopes

Descanned confocal detection systems

Detection via internal descanned detectors

Selection of spectral ranges via internal prism system

Single-detector and dual-detector systems

Optional: Detection via X1 port

High-efficiency H7422 detectors, ultra-fast MCP PMTs, multispectral FLIM modules

Detectors protected by shutter and by overload shutdown

Single-channel and dual-channel (parallel detection) TCSPC systems

On-board image acquisition and time-tag imaging modes
FLIM Electronics integrated in bh Simple-Tau 150, 152, 830 or standard-PC systems

Instrument and data analysis software for Windows 2000, NT, XP, or Vista

Complete microscopes with bh FLIM and bh FLIM upgrades are available from Leica, see link.

FLIM Systems for Olympus FV 1000 Confocal Microscopes

Excitation by bh BDL-405-SMC and BDL-473 SMC picosecond diode lasers

Confocal detection

Direct coupling of FLIM detectors

No coupling loss, ultra-high efficiency

Single-detector systems

Dual-detector systems

Multi-spectral FLIM systems

Detectors protected by overload shutdown

Fiber-coupled SPAD detectors

Single-channel and dual-channel (parallel detection) TCSPC systems

On-board image acquisition and time-tag imaging modes

Electronics integrated in bh Simple-Tau 150, 152, 830 or standard-PC systems

Instrument and data analysis software for Windows 2000, NT, XP, or Vista

Requirements: Fibre inputs for 405 nm or 473 nm lasers must be available
 

Handbook

FLIM Systems for Nikon C1 Scanners

The Nikon C1 scanner uses fibre coupling both for the laser input and for the output to the detector. FLIM upgrades are possible connecting a BDL-SMC laser and a FLIM detector to these fibre connectors. Emission filters are placed directly in front of the FLIM detector. The essential features are:

Excitation by bh BDL-405-SMC, BDL-445-SMC, or BDL-473 SMC picosecond diode lasers

Laser coupled to scan head via single-mode fibre (FC connector)

Fibre coupling of FLIM detectors

Single-detector systems

Dual-detector systems (external beamsplitter used)

Multi-spectral FLIM systems

Detectors protected by overload shutdown

Single-channel and dual-channel (parallel detection) TCSPC systems

On-board image acquisition and time-tag imaging modes

Electronics integrated in bh Simple-Tau 150, 152, 830 or standard-PC systems

Instrument and data analysis software for Windows 2000, NT, XP, or Vista

 

FLIM Systems for Biorad MRC 1024 and Radiance 2000 Multiphoton Microscopes

The Biorad Microscopes have been discontinued in 2004. bh has supplied a number of FLIM systems for these microscopes and still supports existing MRC 1024 and Radiance 2000 FLIM systems.
 

 


Modular FLIM Solutions for other Laser Scanning Microscopes

Due to their modularity the bh can easily be attached to home-built scanning systems. The requirements are:

The system uses a picosecond or femtosecond laser for excitation or

A fibre input for a BDL-SMC picosecond diode laser is available.

The fluorescence light is available at a suitable optical port of the system

Scan clock pulses are available to synchronise the recording with the scanning. For details please see bh TCSPC Handbook and 'Overview about bh Modular FLIM Systems' (below).

 

Additional options for ultra-high speed FLIM

32 MHz throughput

Fully scalable ( 1 to 8 fully parallel TCSPC channels )

Simultaneous detection in all time- and wavelength channels

Data acquisition completely on-board - no transfer bottlenecks

 

 

 

 
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Becker & Hickl GmbH    
Nahmitzer Damm 30
12277 Berlin
Geschäftsführer: Dr. Wolfgang Becker, Helmut Hickl
Amtsgericht Berlin-Charlottenburg HRB 50729
Steuernummer: 29 410 05494
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