FLIM Systems for
Laser Scanning Microscopes
General Purpose FLIM Systems
Data
sheet
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FLIM
Systems for Zeiss LSM 710
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Recording of Z Scans
Multiphoton NDD FLIM
Confocal FLIM
Single-detector systems
Dual-detector systems
Multi-spectral FLIM systems
GaAsP Hybrid
Detectors
Simultaneous detection in all
wavelength channels
Parallel TCSPC systems for
dual-detector systems
Unprecedented count rate
Short acquisition time for
bright samples
Sequential recording mode by
memory swapping
FLIM sequences as fast as 2
images / second
Time-tag and histogram modes
Extremely large images by FIFO
imaging mode
Data sheet,
Handbook
DCS-120 Confocal FLIM
Scanning Systems
Recording of Z Scans
Complete laser scanning FLIM
microscopes
FLIM upgrade for conventional
microscopes
One or two bh BDL-SMC picosecond
diode lasers
Laser wavelengths 405 nm, 440
nm, 473, or 640 nm
Scanning by fast galvano mirrors
Frame times down to <100 ms,
line times down to 1 ms
Frame scan, line scan, and point
(FCS) capability
Two fully confocal detection
channels
Individually selectable pinholes
Individually selectable filters
Secondary beamsplitter wheel
with dichroic and polarising beamsplitters
High efficiency due to
direct-coupled detectors
PMC-100, H7422, ultra-fast MCP
PMT, or SPAD detector modules
Multi-spectral FLIM detector
module
Fully integrated scanning,
detector and laser control
Single-channel and dual-channel
(parallel detection) TCSPC systems
On-board image acquisition and
time-tag imaging modes
Electronics integrated in bh
Simple-Tau 150 or 152 or standard-PC systems
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
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Data sheet,
Overview, Handbook
DCS-120
MACRO
Confocal Scanning FLIM System for
Macroscopic Objects
Confocal scanning principle
Fast scanning galvanometer
mirrors
Excitation by two ps diode
lasers or tuneable ps laser
Recording by bh?s
multidimensional TCSPC technique
High throughput dual-channel
parallel TCSPC architecture
High-efficiency GaAsP hybrid
detectors
Phosphorescence (PLIM) function
included
Compact electronics, controlled
by laptop computer
Data sheet
FLIM
Systems for Zeiss LSM 510 NLO Microscopes
(Multiphoton)
Non-descanned detection
High efficiency, deep-tissue
imaging capability
Single-detector systems
Dual-detector systems
Multi-spectral FLIM systems
PMC-100, H7422, ultra-fast MCP
PMT, multispectral PMT modules
Detectors protected by shutters
and by overload shutdown
Single-channel and dual-channel
(parallel detection) TCSPC systems
On-board image acquisition and
time-tag imaging modes
Electronics integrated in bh
Simple-Tau 150 or 152 or standard-PC systems
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
Upgrades for all LSM 510 NLO
versions, upright or inverted microscopes
Zeiss NDD switch box must be
installed
Data sheet,
Handbook,
Multispectral
detector handbook
FLIM
Systems for Zeiss LSM 510 Microscopes
(One-Photon)
Confocal detection
Excitation by bh BDL-405-SMC
picosecond diode laser
Single-detector systems
Dual-detector systems
Multi-spectral FLIM systems
PMC-100, H7422, ultra-fast MCP
PMT, multispectral PMT, and SPAD detector modules
Detectors protected by overload
shutdown
Single-channel and dual-channel
(parallel detection) TCSPC systems
On-board image acquisition and
time-tag imaging modes
Electronics integrated in bh
Simple-Tau 150, 152, 830 or standard-PC systems
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
Upgrades for all LSM 510
confocal versions, upright or inverted microscopes
Requirements:
Fibre output from LSM 510 scan head must be installed
Input fibre for 405 nm laser must be availableNon-descanned detection
Data sheet,
Handbook,
Multispectral
detector handbook
Non-Descanned FLIM Systems for Leica SP2 and SP5 ( Multiphoton )
Non-descanned detection systems
Detection via RLD port of
microscope
High efficiency, deep-tissue
imaging capability
Single-detector systems and
dual-detector systems
Multi-spectral FLIM systems
PMC-100, H7422, ultra-fast MCP
PMT, multispectral PMT modules
Detectors protected by shutter
and by overload shutdown
Single-channel and dual-channel
(parallel detection) TCSPC systems
On-board image acquisition and
time-tag imaging modes
Electronics integrated in bh
Simple-Tau 150 or 152 or standard-PC systems
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
Requirement: RLD port must be
installed at SP2 or SP5 microscope
Data sheet,
Handbook,
Multispectral
detector handbook
Descanned FLIM Systems for Leica SP2 and SP5 Multiphoton Microscopes
Descanned detection systems
Detection via X1 port of
microscope
High efficiency for thin samples
High suppression of background
light
Single-detector systems and
dual-detector systems
Multi-spectral FLIM systems
PMC-100, H7422, ultra-fast MCP
PMT, multispectral PMT modules
Detectors protected by shutter
and by overload shutdown
Single-channel and dual-channel
(parallel detection) TCSPC systems
On-board image acquisition and
time-tag imaging modes
Electronics integrated in bh
Simple-Tau 150 or 152 or standard-PC systems
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
Handbook,
Multispectral
detector handbook
FLIM Systems for Olympus FV 1000 Confocal Microscopes
Excitation by bh BDL-405-SMC and
BDL-473 SMC picosecond diode lasers
Confocal detection
Direct coupling of FLIM
detectors
No coupling loss, ultra-high
efficiency
Single-detector systems
Dual-detector systems
Multi-spectral FLIM systems
Detectors protected by overload
shutdown
Fiber-coupled SPAD detectors
Single-channel and dual-channel
(parallel detection) TCSPC systems
On-board image acquisition and
time-tag imaging modes
Electronics integrated in bh
Simple-Tau 150, 152, 830 or standard-PC systems
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
Requirements: Fibre inputs for
405 nm or 473 nm lasers must be available
Handbook
FLIM Systems for Nikon C1 Scanners
The Nikon C1 scanner uses fibre
coupling both for the laser input and for the output to the detector. FLIM
upgrades are possible connecting a BDL-SMC laser and a FLIM detector to
these fibre connectors. Emission filters are placed directly in front of the
FLIM detector. The essential features are:
Excitation by bh BDL-405-SMC,
BDL-445-SMC, or BDL-473 SMC picosecond diode lasers
Laser coupled to scan head via
single-mode fibre (FC connector)
Fibre coupling of FLIM detectors
Single-detector systems
Dual-detector systems (external
beamsplitter used)
Multi-spectral FLIM systems
Detectors protected by overload
shutdown
Single-channel and dual-channel
(parallel detection) TCSPC systems
On-board image acquisition and
time-tag imaging modes
Electronics integrated in bh
Simple-Tau 150, 152, 830 or standard-PC systems
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
FLIM Systems for Biorad MRC 1024 and
Radiance 2000 Multiphoton Microscopes
Modular FLIM Solutions
for other Laser Scanning Microscopes
Due to their modularity the bh
can easily be attached to home-built scanning systems. The requirements are:
The system uses a picosecond or
femtosecond laser for excitation or
A fibre input for a BDL-SMC
picosecond diode laser is available.
The fluorescence light is
available at a suitable optical port of the system
Scan clock pulses are available
to synchronise the recording with the scanning. For details please see bh
TCSPC Handbook and 'Overview about bh Modular FLIM Systems' (below).
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