|
| |
|
FLIM Systems
|
FLIM
Systems for Zeiss LSM 710
 |
Multiphoton NDD FLIM
|
|
Confocal FLIM
|
|
Single-detector systems
|
|
Dual-detector systems
|
|
Multi-spectral FLIM systems
|
|
Simultaneous detection in all
wavelength channels
|
|
Parallel TCSPC systems for
dual-detector systems
|
|
Unprecedented count rate
|
|
Short acquisition time for
bright samples
|
|
Sequential recording mode by
memory swapping
|
|
FLIM sequences as fast as 2
images / second
|
|
Time-tag and histogram modes
|
|
Extremely large images by FIFO
imaging mode
|
Data sheet,
Handbook
DCS-120 Confocal FLIM
Scanning Systems
|
Complete laser scanning FLIM
microscopes
|
|
FLIM upgrade for conventional
microscopes
|
|
One or two bh BDL-SMC picosecond
diode lasers
|
|
Laser wavelengths 405 nm, 440
nm, 473, or 640 nm
|
|
Scanning by fast galvano mirrors
|
|
Frame times down to <100 ms,
line times down to 1 ms
|
|
Frame scan, line scan, and point
(FCS) capability
|
|
Two fully confocal detection
channels
|
|
Individually selectable pinholes
|
|
Individually selectable filters
|
|
Secondary beamsplitter wheel
with dichroic and polarising beamsplitters
|
|
High efficiency due to
direct-coupled detectors
|
|
PMC-100, H7422, ultra-fast MCP
PMT, or SPAD detector modules
|
|
Multi-spectral FLIM detector
module
|
|
Fully integrated scanning,
detector and laser control
|
|
Single-channel and dual-channel
(parallel detection) TCSPC systems
|
|
On-board image acquisition and
time-tag imaging modes
|
|
Electronics integrated in bh
Simple-Tau 150 or 152 or standard-PC systems
|
|
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
|
|
  |
|
Data sheet,
Handbook
FLIM
Systems for Zeiss LSM 510 NLO Microscopes
(Multiphoton)
|
Non-descanned detection
|
|
High efficiency, deep-tissue
imaging capability
|
|
Single-detector systems
|
|
Dual-detector systems
|
|
Multi-spectral FLIM systems
|
|
PMC-100, H7422, ultra-fast MCP
PMT, multispectral PMT modules
|
|
Detectors protected by shutters
and by overload shutdown
|
|
Single-channel and dual-channel
(parallel detection) TCSPC systems
|
|
On-board image acquisition and
time-tag imaging modes
|
|
Electronics integrated in bh
Simple-Tau 150 or 152 or standard-PC systems
|
|
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
|
|
Upgrades for all LSM 510 NLO
versions, upright or inverted microscopes
|
|
Zeiss NDD switch box must be
installed
|
Data sheet,
Handbook,
Multispectral
detector handbook
FLIM
Systems for Zeiss LSM 510 Microscopes
(One-Photon)
|
Confocal detection
|
|
Excitation by bh BDL-405-SMC
picosecond diode laser
|
|
Single-detector systems
|
|
Dual-detector systems
|
|
Multi-spectral FLIM systems
|
|
PMC-100, H7422, ultra-fast MCP
PMT, multispectral PMT, and SPAD detector modules
|
|
Detectors protected by overload
shutdown
|
|
Single-channel and dual-channel
(parallel detection) TCSPC systems
|
|
On-board image acquisition and
time-tag imaging modes
|
|
Electronics integrated in bh
Simple-Tau 150, 152, 830 or standard-PC systems
|
|
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
|
|
Upgrades for all LSM 510
confocal versions, upright or inverted microscopes
|
|
Requirements:
Fibre output from LSM 510 scan head must be installed
Input fibre for 405 nm laser must be availableNon-descanned detection
|
Data sheet,
Handbook,
Multispectral
detector handbook
Non-Descanned FLIM Systems for Leica SP2 and SP5 ( Multiphoton )
|
Non-descanned detection systems
|
|
Detection via RLD port of
microscope
|
|
High efficiency, deep-tissue
imaging capability
|
|
Single-detector systems and
dual-detector systems
|
|
Multi-spectral FLIM systems
|
|
PMC-100, H7422, ultra-fast MCP
PMT, multispectral PMT modules
|
|
Detectors protected by shutter
and by overload shutdown
|
|
Single-channel and dual-channel
(parallel detection) TCSPC systems
|
|
On-board image acquisition and
time-tag imaging modes
|
|
Electronics integrated in bh
Simple-Tau 150 or 152 or standard-PC systems
|
|
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
|
|
Requirement: RLD port must be
installed at SP2 or SP5 microscope
|
Data sheet,
Handbook,
Multispectral
detector handbook
Descanned FLIM Systems for Leica SP2 and SP5 Multiphoton Microscopes
|
Descanned detection systems
|
|
Detection via X1 port of
microscope
|
|
High efficiency for thin samples
|
|
High suppression of background
light
|
|
Single-detector systems and
dual-detector systems
|
|
Multi-spectral FLIM systems
|
|
PMC-100, H7422, ultra-fast MCP
PMT, multispectral PMT modules
|
|
Detectors protected by shutter
and by overload shutdown
|
|
Single-channel and dual-channel
(parallel detection) TCSPC systems
|
|
On-board image acquisition and
time-tag imaging modes
|
|
Electronics integrated in bh
Simple-Tau 150 or 152 or standard-PC systems
|
|
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
|
Handbook,
Multispectral
detector handbook
Confocal FLIM Systems for Leica SP2 Microscopes
|
Descanned confocal detection
systems
|
|
Detection via X1 port of
microscope
|
|
Single-detector systems
|
|
Multi-spectral FLIM systems
|
|
PMC-100, H7422, ultra-fast MCP
PMT, multispectral PMT modules
|
|
Detectors protected by shutter
and by overload shutdown
|
|
Single-channel and dual-channel
(parallel detection) TCSPC systems
|
|
On-board image acquisition and
time-tag imaging modes
|
|
Electronics integrated in bh
Simple-Tau 150 or 152 or standard-PC systems
|
|
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
|
|
Requirement: X1 port adaptation
must be installed at SP2 or SP5 microscope
|
Handbook,
Multispectral
detector handbook
Confocal FLIM Systems for Leica SP5 Microscopes
|
Descanned confocal detection
systems
|
|
Detection via internal descanned
detectors
|
|
Selection of spectral ranges via
internal prism system
|
|
Single-detector and
dual-detector systems
|
|
Optional: Detection via X1 port
|
|
High-efficiency H7422 detectors,
ultra-fast MCP PMTs, multispectral FLIM modules
|
|
Detectors protected by shutter
and by overload shutdown
|
|
Single-channel and dual-channel
(parallel detection) TCSPC systems
|
|
On-board image acquisition and
time-tag imaging modes
FLIM Electronics integrated in bh Simple-Tau 150, 152, 830 or standard-PC
systems
|
|
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
|
Complete microscopes with bh FLIM and bh
FLIM upgrades are available from Leica, see
link.
FLIM Systems for Olympus FV 1000 Confocal Microscopes
|
Excitation by bh BDL-405-SMC and
BDL-473 SMC picosecond diode lasers
|
|
Confocal detection
|
|
Direct coupling of FLIM
detectors
|
|
No coupling loss, ultra-high
efficiency
|
|
Single-detector systems
|
|
Dual-detector systems
|
|
Multi-spectral FLIM systems
|
|
Detectors protected by overload
shutdown
|
|
Fiber-coupled SPAD detectors
|
|
Single-channel and dual-channel
(parallel detection) TCSPC systems
|
|
On-board image acquisition and
time-tag imaging modes
|
|
Electronics integrated in bh
Simple-Tau 150, 152, 830 or standard-PC systems
|
|
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
|
|
Requirements: Fibre inputs for
405 nm or 473 nm lasers must be available
|
Handbook
FLIM Systems for Nikon C1 Scanners
|
The Nikon C1 scanner uses fibre
coupling both for the laser input and for the output to the detector. FLIM
upgrades are possible connecting a BDL-SMC laser and a FLIM detector to
these fibre connectors. Emission filters are placed directly in front of the
FLIM detector. The essential features are:
|
|
Excitation by bh BDL-405-SMC,
BDL-445-SMC, or BDL-473 SMC picosecond diode lasers
|
|
Laser coupled to scan head via
single-mode fibre (FC connector)
|
|
Fibre coupling of FLIM detectors
|
|
Single-detector systems
|
|
Dual-detector systems (external
beamsplitter used)
|
|
Multi-spectral FLIM systems
|
|
Detectors protected by overload
shutdown
|
|
Single-channel and dual-channel
(parallel detection) TCSPC systems
|
|
On-board image acquisition and
time-tag imaging modes
|
|
Electronics integrated in bh
Simple-Tau 150, 152, 830 or standard-PC systems
|
|
Instrument and data analysis
software for Windows 2000, NT, XP, or Vista
|
FLIM Systems for Biorad MRC 1024 and
Radiance 2000 Multiphoton Microscopes
|
The Biorad Microscopes have been
discontinued in 2004. bh has supplied a number of FLIM systems for these
microscopes and still supports existing MRC 1024 and Radiance 2000 FLIM
systems.
|
Modular FLIM Solutions
for other Laser Scanning Microscopes
|
Due to their modularity the bh
can easily be attached to home-built scanning systems. The requirements are:
|
|
The system uses a picosecond or
femtosecond laser for excitation or
|
|
A fibre input for a BDL-SMC
picosecond diode laser is available.
|
|
The fluorescence light is
available at a suitable optical port of the system
|
|
Scan clock pulses are available
to synchronise the recording with the scanning. For details please see bh
TCSPC Handbook and 'Overview about bh Modular FLIM Systems' (below).
|
|
|
Additional options for ultra-high speed FLIM
|
32 MHz throughput
|
|
Fully scalable ( 1 to 8 fully
parallel TCSPC channels
)
|
|
Simultaneous detection in all time- and
wavelength channels
|
|
Data acquisition completely
on-board - no transfer bottlenecks
|
|
|
|
|
© 1993-2007
Becker & Hickl GmbH
Nahmitzer Damm 30
12277 Berlin |
Geschäftsführer: Dr. Wolfgang Becker, Helmut Hickl
Amtsgericht Berlin-Charlottenburg HRB 50729
Steuernummer: 29 410 05494
USt-IDNr: 164939770 |
email
info@becker-hickl.com
fon +49 (30) 787 56 32
fax +49 (30) 787 57 34 |
|
|
