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FLIM Systems for
Laser Scanning Microscopes

General Purpose FLIM Systems

Data sheet

  • All microscopes

  • Multiphoton NDD FLIM

  • Confocal FLIM

  • Single-detector systems

  • Dual-detector systems

  • Multi-spectral FLIM systems

 

   FLIM Systems for Zeiss LSM 710           

  • Recording of Z Scans

  • Multiphoton NDD FLIM

  • Confocal FLIM

  • Single-detector systems

  • Dual-detector systems

  • Multi-spectral FLIM systems

  • Phosphorescence lifetime option

  • GaAsP Hybrid Detectors (HPM-100)

  • Simultaneous detection in all wavelength channels

  • Parallel TCSPC systems for dual-detector systems

  • Unprecedented count rate

  • Short acquisition time for bright samples

  • Sequential recording mode by memory swapping

  • FLIM sequences as fast as 2 images / second

  • Time-tag and histogram modes

  • Extremely large images by FIFO imaging mode
     

Data sheet, Handbook

 

  DCS-120 Confocal FLIM Scanning Systems

  • Recording of Z Scans

  • Complete laser scanning FLIM microscopes

  • FLIM upgrade for conventional microscopes

  • PLIM - Phosphorescence lifetime imaging simultaneously with FLIM

  • One or two bh BDL-SMC picosecond diode lasers

  • Laser wavelengths 405 nm, 440 nm, 473, or 640 nm

  • Scanning by fast galvano mirrors

  • Frame times down to <100 ms, line times down to 1 ms

  • Frame scan, line scan, and point (FCS) capability

  • Two fully confocal detection channels

  • Individually selectable pinholes

  • Individually selectable filters

  • Secondary beamsplitter wheel with dichroic and polarising beamsplitters

  • High efficiency due to direct-coupled detectors

  • GaAsP Hybrid Detectors (HPM-100), PMC-100, H7422, ultra-fast MCP PMT, or SPAD detector modules

  • Multi-spectral FLIM detector module

  • Fully integrated scanning, detector and laser control

  • Single-channel and dual-channel (parallel detection) TCSPC systems

  • On-board image acquisition and time-tag imaging modes

  • Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems

  • Instrument and data analysis software for Windows 2000, NT, XP, or Vista

 

 

Data sheet, Overview, Handbook 

 

 

  DCS-120 MACRO

Confocal Scanning FLIM System for Macroscopic Objects

 

  • Confocal scanning principle

  • Fast scanning galvanometer mirrors

  • Excitation by two ps diode lasers or tuneable ps laser

  • Recording by bh´s multidimensional TCSPC technique

  • High throughput dual-channel parallel TCSPC architecture

  • High-efficiency GaAsP hybrid detectors (HPM-100)

  • Phosphorescence (PLIM) function included

  • Compact electronics, controlled by laptop computer

Data sheet

 

 FLIM Systems for Zeiss LSM 510 NLO Microscopes
(Multiphoton)

  • Non-descanned detection

  • High efficiency, deep-tissue imaging capability

  • Single-detector systems

  • Dual-detector systems

  • Multi-spectral FLIM systems

  • High-efficiency GaAsP hybrid detectors (HPM-100)

  • PMC-100, H7422, ultra-fast MCP PMT, multispectral PMT modules

  • Detectors protected by shutters and by overload shutdown

  • Single-channel and dual-channel (parallel detection) TCSPC systems

  • On-board image acquisition and time-tag imaging modes

  • Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems

  • Instrument and data analysis software for Windows 2000, NT, XP, or Vista

  • Upgrades for all LSM 510 NLO versions, upright or inverted microscopes

  • Zeiss NDD switch box must be installed
     

Data sheet, Handbook, Multispectral detector handbook

 

 FLIM Systems for Zeiss LSM 510 Microscopes
 (One-Photon)

  • Confocal detection

  • Excitation by bh BDL-405-SMC picosecond diode laser

  • Single-detector systems

  • Dual-detector systems

  • Multi-spectral FLIM systems

  • High-efficiency GaAsP hybrid detectors (HPM-100)

  • PMC-100, H7422, ultra-fast MCP PMT, multispectral PMT, and SPAD detector modules

  • Detectors protected by overload shutdown

  • Single-channel and dual-channel (parallel detection) TCSPC systems

  • On-board image acquisition and time-tag imaging modes

  • Electronics integrated in bh Simple-Tau 150, 152, 830 or standard-PC systems

  • Instrument and data analysis software for Windows 2000, NT, XP, or Vista

  • Upgrades for all LSM 510 confocal versions, upright or inverted microscopes

  • Requirements:
    Fibre output from LSM 510 scan head must be installed
    Input fibre for 405 nm laser must be availableNon-descanned detection

 




Data sheet, Handbook, Multispectral detector handbook

 

Non-Descanned FLIM Systems for Leica SP2 and SP5 ( Multiphoton )

  • Non-descanned detection systems

  • Detection via RLD port of microscope

  • High efficiency, deep-tissue imaging capability

  • Single-detector systems and dual-detector systems

  • Multi-spectral FLIM systems

  • High-efficiency GaAsP hybrid detectors (HPM-100)

  • PMC-100, H7422, ultra-fast MCP PMT, multispectral PMT modules

  • Detectors protected by shutter and by overload shutdown

  • Single-channel and dual-channel (parallel detection) TCSPC systems

  • On-board image acquisition and time-tag imaging modes

  • Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems

  • Instrument and data analysis software for Windows 2000, NT, XP, or Vista

  • Requirement: RLD port must be installed at SP2 or SP5 microscope
     

      

Data sheet, Handbook, Multispectral detector handbook

 

Descanned FLIM Systems for Leica SP2 and SP5 Multiphoton Microscopes

  • Descanned detection systems

  • Detection via X1 port of microscope

  • High efficiency for thin samples

  • High suppression of background light

  • Single-detector systems and dual-detector systems

  • Multi-spectral FLIM systems

  • PMC-100, H7422, ultra-fast MCP PMT, multispectral PMT modules

  • Detectors protected by shutter and by overload shutdown

  • Single-channel and dual-channel (parallel detection) TCSPC systems

  • On-board image acquisition and time-tag imaging modes

  • Electronics integrated in bh Simple-Tau 150 or 152 or standard-PC systems

  • Instrument and data analysis software for Windows 2000, NT, XP, or Vista

 

Handbook, Multispectral detector handbook

 

 

FLIM Systems for Olympus FV 1000 Confocal Microscopes

  • Excitation by bh BDL-405-SMC and BDL-473 SMC picosecond diode lasers

  • Confocal detection

  • Direct coupling of FLIM detectors

  • No coupling loss, ultra-high efficiency

  • Single-detector systems

  • Dual-detector systems

  • Multi-spectral FLIM systems

  • Detectors protected by overload shutdown

  • Fiber-coupled SPAD detectors

  • Single-channel and dual-channel (parallel detection) TCSPC systems

  • On-board image acquisition and time-tag imaging modes

  • Electronics integrated in bh Simple-Tau 150, 152, 830 or standard-PC systems

  • Instrument and data analysis software for Windows 2000, NT, XP, or Vista

  • Requirements: Fibre inputs for 405 nm or 473 nm lasers must be available
     

Handbook

FLIM Systems for Nikon C1 Scanners

  • The Nikon C1 scanner uses fibre coupling both for the laser input and for the output to the detector. FLIM upgrades are possible connecting a BDL-SMC laser and a FLIM detector to these fibre connectors. Emission filters are placed directly in front of the FLIM detector. The essential features are:

  • Excitation by bh BDL-405-SMC, BDL-445-SMC, or BDL-473 SMC picosecond diode lasers

  • Laser coupled to scan head via single-mode fibre (FC connector)

  • Fibre coupling of FLIM detectors

  • Single-detector systems

  • Dual-detector systems (external beamsplitter used)

  • Multi-spectral FLIM systems

  • Detectors protected by overload shutdown

  • Single-channel and dual-channel (parallel detection) TCSPC systems

  • On-board image acquisition and time-tag imaging modes

  • Electronics integrated in bh Simple-Tau 150, 152, 830 or standard-PC systems

  • Instrument and data analysis software for Windows 2000, NT, XP, or Vista

 

FLIM Systems for Biorad MRC 1024 and Radiance 2000 Multiphoton Microscopes

  • The Biorad Microscopes have been discontinued in 2004. bh has supplied a number of FLIM systems for these microscopes and still supports existing MRC 1024 and Radiance 2000 FLIM systems.
     

 


Modular FLIM Solutions for other Laser Scanning Microscopes

  • Due to their modularity the bh can easily be attached to home-built scanning systems. The requirements are:

  • The system uses a picosecond or femtosecond laser for excitation or

  • A fibre input for a BDL-SMC picosecond diode laser is available.

  • The fluorescence light is available at a suitable optical port of the system

  • Scan clock pulses are available to synchronise the recording with the scanning. For details please see bh TCSPC Handbook and 'Overview about bh Modular FLIM Systems' (below).

 

Additional options for ultra-high speed FLIM

  • 32 MHz throughput

  • Fully scalable ( 1 to 8 fully parallel TCSPC channels )

  • Simultaneous detection in all time- and wavelength channels

  • Data acquisition completely on-board - no transfer bottlenecks

 

 


 

 

©1993-2014 
Becker & Hickl GmbH
Nahmitzer Damm 30
12277 Berlin

Geschäftsführer: Dr. Wolfgang Becker

Amtsgericht Berlin-Charlottenburg HRB 50729
USt-ID Nr: DE164939770
 

email info@becker-hickl.com
fon +49 (30) 212 80 02-0
fax +49 (30) 212 80 02-13