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FLIM for Leica Microscopes

The Leica D FLIM an MP FLIM systems are add-ons of the TCS SP2 and TCS SP5 laser scanning microscopes. The systems are based on the multi-dimensional TCSPC technique and the SPC-830 TCSPC modules of Becker & Hickl. This handbook should be considered a supplement to the handbooks of the Leica TCS SP2, Leica TCS SP5, and the bh TCSPC Handbook. Moreover, we recommend as supplementary literature.

Table of Contents

  • Introduction
  • The Leica FLIM Systems
  • FLIM Measurements
  • Data Analysis
  • Special FLIM System Configurations
  • Applications
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