Back to Handbooks

FLIM Systems for Laser Scanning Microscopes

The FLIM systems are based on bh’s multi-dimensional time-correlated single photon counting (TCSPC) process in combination with confocal or multiphoton scanning by a high-frequency pulsed laser beam. Each photon is characterised by its time in the laser pulse period and the coordinates of the laser spot in the scanning area in the moment of its detection. The recording process builds up a photon distribution over these parameters. The result is an array of pixels, each containing a full fluorescence decay curve in a large number of time channels.

FLIM Functions in Brief

Interactive Scan Control
Any change in the scan area of the microscope immediately becomes effective in the recorded images

Megapixel FLIM Images
With 64 bit SPCM software pixel numbers can be increased to 2048 x 2048 pixels, with a temporal resolution of 256 time channels

Ultra-High Time Resolution
bh multiphoton FLIM systems achieve an instrument response function (IRF) of less than 20 ps FWHM

And more
View the document below for more details

Table of Contents

  • General Features
  • FLIM Functions in Brief
  • bh FLIM Systems for Various Microscopes
  • FLIM Systems for Clinical Imaging
  • FLIM for NSOM Systems
  • FLIM for other Scanning Systems

© 2019 Becker & Hickl GmbH. All rights reserved.

Privacy PolicyImprint