The FLIM systems for the Zeiss LSM 710 /780 / 880 family laser scanning microscopes are based on bh’s Multi-Dimensional TCSPC technique and 64 bit Megapixel technology. The systems feature single-photon sensitivity, excellent spatial and temporal resolution, multi-exponential decay analysis, and short acquisition time. The systems are compatible with multiphoton excitation, diode laser excitation, and tuneable excitation by the Zeiss Intune laser. The system fully exploits the fast beam scanning capability of the Zeiss LSM.
The functions include advanced techniques like multi-wavelength detection, Z stack FLIM, time-series FLIM, spatial and temporal mosaic FLIM, fluorescence lifetime-transient scanning (FLITS), and phosphorescence lifetime imaging (PLIM).
We describe the use of the system for the measurement of molecular environment parameters, FRET measurements, autofluorescence FLIM of tissue, and plant physiology.
Table of Contents
- Principle of the bh FLIM Technique
- Components of the LSM 710/780/880 FLIM Systems
- SPCM Software
- System Parameters for Basic FLIM Experiments
- Advanced FLIM Techniques and Procedures
- A Few Things FLIM Users Should Know
- SPCImage Data Analysis Software
- System Setup
This brochure is a condensed version of the handbook of the bh FLIM systems for the Zeiss LSM series microscopes.