The system uses the routing function of multidimensional TCSPC to record several lifetime imges detected by separate detectors in a single TCSPC module. A 'Router' combines the photon pulses of all detectors into a common photon pulse line. Simultaneously, it generates a 'Channel' signal that indicates in which of the detectors a particular photon was detected. Both signals are sent into the TCSPC module. For every photon, the TCSPC module determines the time in the laser pulse sequence, the number of the detetcor channel in which it was detected, and the location of the laser beam in the sample in the moment when the photon has been detected. From these pieces of information, the TCSPC process builds up a photon distribution over the times of the photons in the laser pulse period, the numbers of the detector channels in which the photons were detected, and the location, y,x, in the sample. The distribution resembles a stack of lifetime images, each of which is associated to one of the detectors.
A large number of FLIM systems in the routing configuration are still in use. The disadvantage is that the throughput rate is that of one TCSPC module, and that overload in one channel can cause artefacts in other channels. Routing systems for two, three, and four detectors are therefore more and more replaced with multi-module FLIM systems.
- Standard FLIM in Several Wavelength Channels
- FRET Imaging
- Imaging of Molecular Environment Parameters
- NADH FLIM
- Anisotropy Decay Measurements
- Single-Molecule Spectroscopy
- FCS and FCCS Systems