SPC-130-EMN

Low-Cost TCSPC Module
  • Time Channel Width Down to 813 fs
  • Electrical Time Resolution 2.5 ps RMS (6.6 ps FWHM)
  • 10 MHz Saturated Count Rate
  • Fluorescence Lifetime Recording
  • Phosphorescence Lifetime Recording
  • Free Instrument Software for Windows Vista/7/8/10 (Realtime Calculation and Fitting of FCS Curves)
  • Parallel Operation of Up to 4 Modules
  • Available as Multi-Module Package e.g. SPC-132-EMN, SPC-133-EMN and SPC-134-EMN

The SPC-130-EMN is a small size and low-cost time-correlated single photon counting (TCSPC) module (patented) with high-time resolution for standard fluorescence lifetime applications and for applications requiring a large number of parallel TCSPC channels. The board offers a PCI interface and can be easily installed in nearly all PCs.

General Information

The SPC-130-EMN boards have all standard photon distribution modes, the continuous-flow mode, and the FIFO (time-tag) mode implemented. However, this board has no imaging mode. The SPC-130-EMN board can thus be used for traditional fluorescence lifetime experiments, diffuse optical tomography, stopped flow experiments, single molecule detection and combined FCS/lifetime experiments. The SPC-130-EMN has especially overvoltage-hardened signal inputs. They are reliable and rugged, and can be used even under harsh conditions. When ordered in large quantities for OEM applications, the bh SPC-130-EMN modules offer unprecedented price-performance ratio. The module is upgradable to SPC-150N for fluorescence lifetime imaging microscopy (FLIM) and other laser scanning applications.

Measurement Software Included

The SPCM operating and measurement software is included with all SPC series modules. SPCM provides online calculation and display (2D, 3D) of data (decay curves, FCCS) acquired in multiple operation modes. SPCM software undergoes active continuous development. SPCM receives frequent updates with new features and bug fixes. Read more…

FLIM and FCS Data Analysis

For advanced fluorescence lifetime single-curve data analysis, please use SPCImage.
For advanced fluorescence correlation spectroscopy (FCS) and cross-correlation (FCCS) data analysis, please use Burst Analyzer.

Custom Programming Libraries (DLL, LabVIEW)

For automation and custom software integration, DLLs and LabVIEW drivers are available. Read more…

Photon Channel

 

Principle

Constant Fraction Discriminator (CFD)

Discriminator Input Bandwidth

4 GHz

Time Resolution (FWHM/RMS, electr.)

6.6 ps / 2.5 ps

Variance in Time of IRF max.

< 1 ps over 50 s

Optimum Input Voltage Range

-30 mV to -500 mV

Min. Input Pulse Width

200 ps

Threshold

0 to -250 mV

Zero Cross Adjust

-100 mV to 100 mV

Synchronisation Channel

 

Principle

Constant Fraction Discriminator (CFD)

Discriminator Input Bandwidth

4 GHz

Optimum Input Voltage Range

-30 mV to -500 mV

Min. Input Pulse Width

200 ps

Threshold

0 to -250 mV

Frequency Range

0 to 150 MHz

Frequency Divider

1, 2, 4

Zero Cross Adjust

-100 mV to 100 mV

Time-to-Amplitude Converters / ADCs

 

Principle

Ramp Generator / Biased Amplifier

TAC Range

50 ns to 5 µs

Biased Amplifier Gain

1 to 15

Biased AmplifierOffset

0 % to 50 % of TAC Range

Time Range incl. Biased Amplifier

3.3 ns to 5 µs

Min. Time Channel Width

813 fs

ADC Principle

Flash ADC with Error Correction

Diff. Nonlinerarity

< 0.5 % RMS, typ. < 1 % peak-peak

Data Acquisition

Histogram Mode

Method

on-board 2-dim. histogramming process

Online Display

Decay curves (waveforms)

Dead Time

100 ns, independent of computer speed

Saturated Count Rate

10 MHz

Sustained Count Rate

10 MHz

Max. useful Count Rate (50 % loss)

5 MHz

Max. Number of curves in memory

65536

16384

4096

1024

256

64

Number of Time Channels / Curve

4

16

64

256

1024

4096

Max. Counts / Channel

16 bits

Overflow Control

none, stop, repeat and correct

Collection Time

0.1 µs to 100,000 s

Diplay Interval Time

10 ms to 100,000 s

Repeat Time

0.1 µs to 100,000 s

Curve Control (Internal Sequencing)

Programmable Hardware Sequencer

Input Curve Control (Routing)

4 bit, TTL

Input Count Enable Control

1 bit, TTL

Input External Event Markers

4 bit, TTL

Input Experiment Trigger

TTL

Data Acquisition

FIFO / Parameter-Tag Mode

Method

Time and wavelength tagging of individual photons and continuous writing to disk

Online Display

Decay functions, FCS, Cross-FCS, PCH MCS Traces

FCS Calculation

Multi-tau algorithm, online calculation and online fit

Number of counts of decay/ waveform recording

unlimited

Dead Time

100 ns

Saturated Count Rate, Peak

10 MHz

Sustained Count Rate (bus transfer limit)

typ. 4 MHz

Output Data Format (ADC / Macrotime / Routing)

12 / 12 / 4

FIFO Buffer Capacity (Photons)

2 * 106

Macro Timer Resolution, Internal Clock

50 ns, 12 bit, overflows marked by MOTF entry in data stream

Input Macro Timer Resolution, Clock from Sync

10 ns to 100 ns, 12 bit, overflow marked by MOTF entry in data stream

Input Curve Control (external Routing)

4 bit, TTL

Input Count Enable Control

1 bit, TTL

Input Experiment Trigger

TTL

Operation Environment

 

PC System

Windows 7 to 10, > 8 GB RAM, 64 bit operating system recommended

PC Interface

PCI

Power Consumption

approx. 12 W from +5 V, 0.7 W from +12 V

Dimensions

240 mm x 130 mm x 15 mm

 

The bh TCSPC Handbook
8th edition, corrected and updated September 2019

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