SPC-150NX

High-Resolution Version of SPC-150N
  • Time Channel Width Down to 407 fs
  • Electrical Time Resolution 1.6 ps RMS (3.5 ps FWHM)
  • 10 MHz Saturated Count Rate
  • FLIM, PLIM, FLITS, FCS, Mosaic FLIM
  • Improved Resolution for Ultra-Fast Detectors
  • Free Instrument Software for Windows Vista/7/8/10 (Realtime Calculation and Fitting of FCS Curves)
  • Parallel Operation of Up to 4 Modules
  • Available as Multi-Module Package e.g. SPC-152NX, SPC-153NX and SPC-154NX

The SPC-150NX is a resolution-enhanced version of the SPC-150N for ultra-fast detectors. The board can be used for traditional fluorescence lifetime measurements, single molecule spectroscopy, FCS and FCCS recording, FLIM, Mosaic FLIM, FLITS, and combined FLIM / PLIM applications. The SPC-150NX is recommended especially for applications that require ultimate time resolution from superconducting detectors, MCP PMTs, and fast hybrid detectors. The board offers a PCI interface and can be easily installed in nearly all PCs.

General Information

The SPC-150NX boards is a resolution-enhanced version of the SPC-150N. The minimum time-channel width is 405 fs. These modules are recommended especially for applications that require ultimate time resolution from superconducting detectors, MCP PMTs, and fast hybrid detectors. The SPC-150NX achieves sub-20 ps (FWHM) IRF width with these detectors. The module offers all standard photon distribution modes, the continuous-flow mode, the FIFO (time-tag) mode and the FIFO Imaging (FLIM) mode is implemented. The SPC-150NX board can thus be used for traditional fluorescence lifetime experiments, diffuse optical tomography, stopped flow experiments, single molecule spectroscopy, FCS and FCCS recording, FLIM, Mosaic FLIM, FLITS, and combined FLIM / PLIM. One or more SPC-150NX modules can be used for the bh DCS-120 confocal FLIM system. They can be also the basis of the standard FLIM systems for the Zeiss LSM 710 and 510 microscopes and various other confocal and multiphoton laser scanning microscopes.

Measurement Software Included

The SPCM operating and measurement software is included with all SPC series modules. SPCM provides online calculation and display (2D, 3D) of data (decay curves, FLIM, FCCS) acquired in multiple operation modes. SPCM software undergoes active continuous development. SPCM receives frequent updates with new features and bug fixes. Read more…

FLIM and FCS Data Analysis

For advanced fluorescence lifetime imaging (FLIM) and single-curve data analysis, please use SPCImage.
For advanced fluorescence correlation spectroscopy (FCS) and cross-correlation (FCCS) data analysis, please use Burst Analyzer.

Custom Programming Libraries (DLL, LabVIEW)

For automation and custom software integration, DLLs and LabVIEW drivers are available. Read more…

Photon Channel

 

Principle

Constant Fraction Discriminator (CFD)

Discriminator Input Bandwidth

4 GHz

Time Resolution (FWHM/RMS, electr.)

< 3.5 ps / 1.6 ps

Variance in Time of IRF centroid

< 0.4 ps RMS over 100 s

Optimum Input Voltage Range

-30 mV to -500 mV

Min. Input Pulse Width

200 ps

Threshold

0 to -250 mV

Zero Cross Adjust

-100 mV to 100 mV

Synchronisation Channel

 

Principle

Constant Fraction Discriminator (CFD)

Discriminator Input Bandwidth

4 GHz

Optimum Input Voltage Range

-30 mV to -500 mV

Min. Input Pulse Width

200 ps

Threshold

0 to -250 mV

Frequency Range

0 to 150 MHz

Frequency Divider

1, 2, 4

Zero Cross Adjust

-100 mV to 100 mV

Time-to-Amplitude Converters / ADCs

 

Principle

Ramp Generator / Biased Amplifier

TAC Range

25 ns to 2.5 µs

Biased Amplifier Gain

1 to 15

Biased Amplifier Offset

0 % to 50 % of TAC Range

Time Range incl. Biased Amplifier

1.67 ns to 2.5 µs

Min. Time Channel Width

407 fs

ADC Principle

50 ns Flash ADC with Error Correction

Diff. Nonlinerarity

< 0.5 % RMS, typ. < 1 % peak-peak

Data Acquisition

Histogram Mode

Method

on-board multi-dim. histogramming process

Dead Time

100 ns, independent of computer speed

Saturated Count Rate

10 MHz

Max. Counts / Time Channel

16 bits

Overflow Control

none, stop, repeat and correct

Collection Time

0.1 µs to 100,000 s

Diplay Interval Time

10 ms to 100,000 s

Repeat Time

0.1 µs to 100,000 s

Sequencing Recording

Programmable Hardware Sequencer, unlimited recording by Memory swapping, in curve mode and scan mode

Synchronisation with Scanning

Pixel, Line and Frame from Scanning Device

Routing

7 bit, TTL

Experiment Trigger

TTL

Data Acquisition

FIFO / Parameter-Tag Mode

Method

Time and wavelength tagging of individual photons and continuous writing to disk

Online Display

Decay functions, FCS, Cross-FCS, PCH MCS Traces

FCS Calculation

Multi-tau algorithm, online calculation and online fit

Number of counts of decay/ waveform recording

unlimited

Dead Time

100 ns

Saturated Count Rate, Peak

10 MHz

Sustained Count Rate (bus transfer limit)

typ. 4 MHz

Max. Counts / Time Channel (Counting Depth)

unlimited

Output Data Format (ADC / Macrotime / Routing)

12 / 12 / 4

FIFO Buffer Capacity (Photons)

2 * 106

Macro Timer Resolution, Internal Clock

50 ns, 12 bit, overflows marked by MOTF entry in data stream

Input Macro Timer Resolution, Clock from Sync

10 ns to 100 ns, 12 bit, overflow marked by MOTF entry in data stream

Input Curve Control (external Routing)

4 bit, TTL

External Event Markers

4 bit, TTL

Enable Count Control

1 bit, TTL

Input Experiment Trigger

TTL

Data Acquisition

FIFO / Parameter-Tag Imaging Mode

Method

Buildingup images from time- and wavelength tagged data

Online Display

up to 8 Images in different time and wavelength windows

Synchronisation with Scanner

via Frame Clock, Line Clock and Pixel Clock Pulses

Detector / Wavelength Channels

1 to 16

Image resolution (64-bit SPCM Software)

 

No. of Time Channels

64

256

1024

4096

No. of Pixels, 1 Detector Channel

4096 x 4096

2048 x 2048

1024 x 1024

512 x 512

No. of Pixels, 16 Detector Channels

1024 x 1024

512 x 512

256 x 256

128 x 128

Operation Environment

 

PC System

Windows 7 to 10, > 8 GB RAM, 64 bit operating system recommended

PC Interface

PCI

Power Consumption

approx. 12 W from +5 V, 0.7 W from +12 V

Dimensions

240 mm x 130 mm x 15 mm

 

The bh TCSPC Handbook
7th edition, corrected and updated September 2017

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