BH FLIM systems are capable of recording FLIM at frame rates of 20 fps or more. Data can be recorded by bh’s Express FLIM Technology or, alternatively, by recording a continuous stream of single-photon data from a sample scanned by a fast scanner and extracting subsequent frames from this data stream. The video sequence below demonstrates FLIM at a beating mouse organoid heart.
The data were recorded with a standard bh TCSPC FLIM system integrated in a Zeiss LSM 980 laser scanning microscope. Data acquisition and experiment control was performed by a combination of bh FLIM software with Zeiss ZEN software, transformation into an MP4 video sequence by bh SPCDynamcis.
