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DCS-120 System Scans Well Plates

In 2017 the bh DCS-120 confocal FLIM system has been upgraded by a software-controlled motor stage [1]. In this configuration, the system is able to scan well plates. The well plate is placed on the motor stage. A defined area within the first well is scanned by the optical scanner of the DCS system, then the operating software shifts the plate by the distance between the wells, and scans the next well. The advantage of confocal scanning is that data are obtained from a thin focal plane inside the wells. Therefore, the decay curves are not impaired by re-absorption and re-emission. Moreover, possible effects of rotational depolarisation are cancelled by the high NA of the microscope lens. The data are saved as a normal Mosaic FLIM-mode file of the SPCM operating software [2]. A preliminary liftime image is displayed during the acquisition. Precision multi-exponential lifetime analysis is performed by bh SPCImage data analysis software [1]. For details of the recording principle and motor stage operation please see:

1. DCS-120 Confocal and Multiphoton FLIM Systems, User handbook, 7th edition (2017).

2. The bh TCSPC Handbook, 7th edition (2017).

Figures: Well plate on microscope stage (left), fluorescence-lifetime image (middle) and fluorescence decay curves from two wells (right).

September 9, 2018

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