Application Notes

Two-Photon Metabolic FLIM with the Coherent Axon 780 Fibre Laser

Fast GPU-Based Global Fit of TCSPC FLIM Data

Metabolic FLIM with Simultaneous pH Imaging

High Resolution Z-Stack FLIM with the Becker & Hickl DCS-120 Confocal FLIM System

Two-Photon FLIM System with a Small Femtosecond Fibre Laser

SPC-QC-104: Precision FLIM and Fast FLIM in One

High-Resolution LIDAR with the SPC-QC-104

Measurement of Membrane Potentials in Cells by TCSPC FLIM

Double-Exponential FLIM-FRET Approach is Free of Calibration

A Common Mistake in Lifetime-Based FRET Measurement

High-Resolution Multiphoton FLIM Reveals Ultra-Fast Fluorescence Decay in Human Hair

Suppression of Lens Fluorescence in FLIO Images of Cataract Patients

8.7 ps FWHM IRF Width from Ultrafast SPAD

Label-Free Multiphoton FLIM of Moving Bacteria

Metabolic FLIM of Macroscopic Objects

Ultra-Fast Fluorescence Decay in Malignant Melanoma

Ultra-Fast Fluorescence Decay in Natural Carotenoids

Ultra-Fast Fluorescence Decay in Scottish Whisky

FLIM at a Time-Channel Width of 300 Femtoseconds

A Differential Discriminator for bh TCSPC Modules

DCS-120 FLIM System Detects FMN in Live Cells

Lifetime-Intensity Mode Delivers Better FLIM Images

273 ps FWHM TCSPC Response with Hamamatsu H15620 NIR PMT

Two-Photon FLIM of Pollen Grains Reveals Ultra-Fast Decay Component

Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO)

High-Resolution Measurement of NADH and FAD Fluorescence Decay with the DCS-120 MP

Two-Photon FLIM of Mushroom Spores Reveals Ultra-Fast Decay Component

FLIM with Excitation-Wavelength Multiplexing

Two-Photon FLIM with a Femtosecond Fibre Laser

Influence of Magnetic Fields on the IRF of High-Speed Detectors for TCSPC

Precision Fluorescence-Lifetime Imaging of a Moving Object

FLIO Data Acquisition and Analysis – The Road to Success

Shifted-Component Model Improves FLIO Data Analysis

4.4 ps IRF width of TCSPC with an NbN Superconducting Nanowire Single Photon Detector

Software-Integrated FLIM for Nikon A1+ Confocals

Metabolic Imaging with the DCS-120 Confocal FLIM System: Simultaneous FLIM of NAD(P)H and FAD

TCSPC System Records FLIM of a Rotating Object

New SPCM Version 9.78 Comes With New Software Functions

Fast-Acquisition Multiphoton FLIM with the Zeiss LSM 880 NLO

Fast-Acquisition TCSPC FLIM: What are the Options?

Fast-Acquisition TCSPC FLIM System with sub-25 ps IRF Width

DCS-120 FLIM System Records X-Y Mosaics

Ultra-fast HPM Detectors Improve NAD(P)H FLIM

Sub-20ps IRF Width from Hybrid Detectors and MCP-PMTs

New SPCImage Version Combines Time-Domain Analysis with Phasor Plot

Wide-Field TCSPC FLIM with bh SPC-150N TCSPC System and Photek FGN 392-1000 Detector

bh TCSPC Systems Record FLIM with Sutter MOM Microscopes

SPC Modules Record FLIM with Bidirectional Scanning

SPCM Software Runs Online-FLIM at 10 Images per Second

bh – Abberior Combination Records STED FLIM at Megapixel Resolution

Online STED FLIM with bh – Abberior Combination

Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation

World Record in TCSPC Time Resolution: Combination of bh SPC-150NX with SCONTEL NbN Detector yields 17.8 ps FWHM

80 ps FHWM Instrument Response with ID230 InGaAs SPAD and SPC-150 TCSPC Module

DCS-120 MP System Records Multiphoton FLIM and PLIM

The PZ-FLIM-110 Piezo-Scanning FLIM System

bh FLIM Systems Record Calcium Transients in Live Neurons

TCSPC Fibre-Probe System with an Exchangeable Tip

Implantable Fibre-Optical Fluorescence-Lifetime Detection System for in-vivo Applications

NSOM FLIM with the Nanonics AFM/NSOM System

An AFM/NSOM System with Fluorescence Lifetime Imaging

Zeiss BiG-2 GaAsP Detector is Compatible with bh FLIM Systems

Mosaic FLIM: New Dimensions in Fluorescence Lifetime Imaging

FLIM and FCS by Pulse-Interleaved Excitation with the Zeiss LSM 710/780 Intune System

Multiphoton NDD FLIM at NIR Detection Wavelengths with the Zeiss LSM 7MP and OPO Excitation

SPC-160 Delivers Linear Intensities in High Count Rate FLIM Applications

Multiphoton FLIM with the Leica HyD RLD Detectors

Megapixel FLIM with bh TCSPC Modules – The New SPCM 64-bit Software

TCSPC at Wavelengths from 900 nm to 1700 nm

DCS-120 Confocal Scanning System: FLIM with NIR Dyes

DCS-120 Confocal Scanning FLIM System: Two-Photon Excitation with Non-Descanned Detection

Combined Fluorescence and Phosphorescence Lifetime Imaging (FLIM / PLIM) with the Zeiss LSM 710 NLO Microscopes

Spatially Resolved Recording of Fluorescence-Lifetime Transients by Line- Scanning TCSPC

DCS-120 Confocal FLIM System with Wideband Beamsplitter

FLIM of Macroscopic Objects: Imaging in the Primary Image Plane of the DCS-120 Scanner

Microsecond Decay FLIM: Combined Fluorescence and Phosphorescence Lifetime Imaging

Multiphoton Multiwavelength NDD FLIM with Zeiss LSM 710 NLO

Non-Descanned FLIM Detection in Multiphoton Microscopes

The HPM-100-50 Hybrid Detector: Increased Dynamic Range for DOT

An 8-Channel Parallel Multispectral TCSPC FLIM System

Tuneable Excitation FLIM with the LSM 710 Intune System

Recording Z Scans with the DCS-120 Confocal Scanning FLIM System

FLIM Systems for Zeiss LSM 710 Record Z Stacks

The HPM-100-40 Hybrid Detector

Recording the Instrument Response Function of a Multiphoton FLIM System

Non-Descanned FLIM Systems for Olympus FV-1000 and FV-300 Multiphoton Microscopes

bh FLIM Systems for Nikon C1 Scanners

Make Life Easier by Using Modern Features of the SPCM Software

NDD FLIM Systems for Leica SP2 MP and SP5 MP Multiphoton Microscopes

Time-Tag Recording: A New Old Feature of the bh SPC Cards

FLIM in the FIFO Imaging Mode: Large Images with Small TCSPC Modules

Timing Stability of TCSPC Experiments

Recording the Kautsky Effect by Fluorescence Lifetime Detection

Two-Photon Fluorescence Excitation by Picosecond Diode Lasers

Detectors for High-Speed Photon Counting

Setting up High Gain Detector Electronics for TCSPC

How (and why not) to Amplify PMT Signals

The Event Mode of the PMS-300 Multiscaler

bh Application Notes – The Knowledge Base for Users of TCSPC, Fluorescence Lifetime, FLIM, Molecular Imaging, and NIRS Techniques

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