FLIM operation with the bh TCSPC/FLIM modules has been implemented in the new ‘NIS-Elements’ software of the Nikon A1+ confocal laser confocal laser scanning microscopes. The systems use one to four bh BDS-SM picosecond diode lasers, up to four bh HPM-100 hybrid detectors, and up to four fully parallel SPC-150N TCSPC data recording channels. All FLIM components are controlled via Nikon’s NIS-Elements software. Intensity images, FLIM images, and decay curves from regions of interest are displayed online. For precision multi-exponential data analysis, the system incorporates bh’s SPCImage FLIM data analysis software.
Keywords: FLIM, Confocal Microscope, Fluorescence Decay, Molecular Imaging, FRET, Metabolic Imaging