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bh – Abberior Combination Records STED FLIM at Megapixel Resolution

The combination of the Abberior STED system with the bh Simple-Tau 150/154 TCSPC FLIM system records FLIM data at a spatial resolution of better than 40 nm. The image format can be as large as 2048 x 2048 pixels, with 256 time channels per pixel. An image area of 40 x 40 micrometers can thus be covered with 20 nm pixel size, fully satisfying the Nyquist criterion. The system benefits from Windows 64 bit technology used both in the Abberior and in the bh data acquisition software, from the combined processing power of the two system computers, and the high data throughput of up to four parallel TCSPC FLIM channels. The system achieves peak count rates in excess of 5 MHz per FLIM channel, resulting in unprecedented signal-to-noise ratio and short acquisition time. For uncompressed image please see application note ‘bh – Abberior Combination Records STED FLIM at Megapixel Resolution‘.

Tubulin fibres in a BPAE cell. Use Adobe zoom function to see image at full resolution.

May 23, 2016

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