Using new 64 bit SPCM data acquisition software, the bh DCS‑120 FLIM system records images of the full field of view of the microscope lens at diffraction-limited resolution. Image formats of up to 2048 x 2048 pixels with 256 time channels per pixel can be used. Two such images are recorded simultaneously in the two parallel channels of the DCS‑120 system. Megapixel FLIM is extremely useful for tissue imaging, and when FLIM data of a large number of cells have to be compared. FLIM data of all cells in the fields of view are obtained simultaneously, and under perfectly identical experimental conditions. The results are therefore exactly comparable. For details please see: Confocal FLIM System and Confocal Scanning FLIM Systems, User Handbook.
FLIM of BPAE sample measured with DCS-120 Confocal Scanning FLIM System with two SPC-150 TCSPC FLIM modules and HPM-100-40 GaAsP hybrid detectors. Excitation wavelength 470 nm, detection wavelength 495 to 560 nm (left) and 560 nm to 650 nm (right), 2048 x 2048 pixels, 256 time channels per pixel.