The FLIM systems for the Zeiss LSM 710 /780 / 880 family laser scanning microscopes are based on bh’s Multi-Dimensional TCSPC technique and 64 bit Megapixel technology. The systems feature single-photon sensitivity, excellent spatial and temporal resolution, multi-exponential decay analysis, and short acquisition time. The systems are compatible with multiphoton excitation, diode laser excitation, and tuneable excitation by the Zeiss Intune laser. The system fully exploits the fast beam scanning capability of the Zeiss LSM. The functions include advanced techniques like multi-wavelength detection, Z stack FLIM, time-series FLIM, spatial and temporal mosaic FLIM, fluorescence lifetime-transient scanning (FLITS), and phosphorescence lifetime imaging (PLIM). We describe the use of the system for the measurement of molecular environment parameters, FRET measurements, autofluorescence FLIM of tissue, and plant physiology.
For LSM 980 FLIM systems please see addendum, FLIM Systems for Zeiss LSM 980 Microscopes.
Keywords: FLIM, PLIM, TCSPC, Confocal Microscope, Multiphoton Microscope, Molecular Imaging, FRET, Metabolic Imaging