With version 9.72 64-bit SPCM software, bh FLIM systems record and display fluorescence lifetime images at a rate of up to 10 images per second. The data are recorded by bh’s multi-dimensional TCSPC technique in combination with confocal or multiphoton laser scanning, the images are created by first-moment analysis. The technique combines near-ideal photon efficiency with short acquisition and calculation times. It works for all SPC-150, SPC-150N, SPC-160, and SPC-830 FLIM systems that use fast scanning. The figure below shows online-FLIM images recorded with 128×128 pixels at a rate of 10 images per second (left) and with 256×256 pixels at a rate of 2 images per second (right). Read more in application note: SPCM Software Runs Online-FLIM at 10 Images per Second
Left: 128x128-pixel images. Acquisition time 0.1 s, binning 3x3 pixels. Right: 256x256-pixel images. Acquisition time 0.5 s, binning 3x3 pixels.