Advanced time-correlated single photon counting (TCSPC) and picosecond diode lasers were used to record light-induced transients of the fluorescence lifetime of chlorophyll in living plants. The experiment setup is almost free of optical components and essentially built up from commercially available lasers, detectors, and TCSPC modules. Both photochemical quenching and non-photochemical quenching transients are resolved, with a resolution of 100 μs per decay curve.
Back to Application Notes