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Recording the Kautsky Effect by Fluorescence Lifetime Detection

Advanced time-correlated single photon counting (TCSPC) and picosecond diode lasers were used to record light-induced transients of the fluorescence lifetime of chlorophyll in living plants. The experiment setup is almost free of optical components and essentially built up from bh picosecond diode lasers, photon detectors, and TCSPC devices. Both photochemical quenching and non-photochemical quenching transients are resolved, with a resolution of 100 μs per decay curve.

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