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Tuneable Excitation FLIM with the LSM 710 Intune System

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Tuneable-excitation FLIM uses the ‘In Tune’ laser of the Zeiss LSM 710 system. By tuning the excitation wavelength to exactly the absorption maximum of the fluorophores maximum efficiency is obtained at minimum photobleaching. High laser power, excellent spectral purity of the laser, high efficiency of the confocal detection path, combined with the high efficiency of the bh GaAsP hybrid detectors result in FLIM results of excellent accuracy.

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