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Tuneable Excitation FLIM with the LSM 710 Intune System

We describe tuneable-excitation FLIM with a bh TCSPC FLIM system combined with a Zeiss LSM 710 Intune laser scanning system. The tuneable-excitation FLIM system uses the ‘In Tune’ laser of the Zeiss LSM 710 as an excitation source. By tuning the excitation wavelength to exactly the absorption maximum of the fluorophores maximum efficiency is obtained at minimum photobleaching. High laser power, excellent spectral purity of the laser, high efficiency of the confocal detection path, combined with the high efficiency of the bh GaAsP hybrid detectors result in FLIM results of excellent accuracy.

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