SPC-160 / SPC-160PCIe

TCSPC Imaging, Multiscaler Imaging and FCS Module
  • Time Channel Width Down to 813 fs
  • Electrical Time Resolution 2.5 ps RMS (6.6 ps FWHM )
  • 12.5 MHz Saturated Count Rate
  • Fast Parallel Counter Channel for FLIM Intensities
  • High-Speed Interface for Laser Scanning Microscopes
  • Free Instrument Software for Windows Vista/7/8/10 (Realtime Calculation and Fitting of FCS Curves)
  • Parallel Operation of Up to 4 Modules
  • Available as Multi-Module Package e.g. SPC-162, SPC-163 and SPC-164
  • Available as PCI Express Version (SPC-160PCIe)

The SPC-160 is a high-end performance time-correlated single photon counting (TCSPC) imaging and imaging multiscaler module with high-time resolution. The board can be used for traditional fluorescence lifetime measurements, single molecule spectroscopy, FCS and FCCS recording, FLIM, Mosaic FLIM, FLITS, and combined FLIM / PLIM applications. They can be also the basis of FLIM upgrades for various scanning microscopes. The board offers a PCI interface and can be easily installed in nearly all PCs. It is also available as a PCI express version (SPC-160PCIe).

General Information

The SPC-160/SPC-160PCIe boards have a large internal memory and a fast bus interface, combined with the largest set of functions and operation modes of all bh TCSPC modules. They have the fast discriminators of the SPC-150N. Moreover, they have an additional parallel photon counting channel in the FIFO Imaging (FLIM) mode. The 160 modules are thus able to record intensity images up to a detector peak count rate of about 100 MHz. Intensity information from the parallel counter channel and lifetime information from the TCSPC imaging channel are combined to obtain FLIM images that have a linear intensity scale up to extremely high-count rates. As the SPC-150N, the SPC-160/SPC-160PCIe modules can be used for traditional fluorescence lifetime experiments, diffuse optical tomography, stopped flow experiments, single molecule spectroscopy, FCS and FCCS recording, FLIM, multi-wavelength FLIM, Mosaic FLIM, and simultaneous FLIM / PLIM.

Measurement Software Included

The SPCM operating and measurement software is included with all SPC series modules. SPCM provides online calculation and display (2D, 3D) of data (decay curves, FLIM, FCCS) acquired in multiple operation modes. SPCM software undergoes active continuous development. SPCM receives frequent updates with new features and bug fixes. Read more…

FLIM and FCS Data Analysis

For advanced fluorescence lifetime imaging (FLIM) and single-curve data analysis, please use SPCImage.
For advanced fluorescence correlation spectroscopy (FCS) and cross-correlation (FCCS) data analysis, please use Burst Analyzer.

Custom Programming Libraries (DLL, LabVIEW)

For automation and custom software integration, DLLs and LabVIEW drivers are available. Read more…

Photon Channel

 

Principle

Constant Fraction Discriminator (CFD)

Discriminator Input Bandwidth

4 GHz

Time Resolution (FWHM/RMS, electr.)

6.6 ps / 2.5 ps

Variance in Time of IRF max.

< 1 ps over 50 s

Optimum Input Voltage Range

-30 mV to -500 mV

Min. Input Pulse Width

200 ps

Threshold

0 to -250 mV

Zero Cross Adjust

-100 mV to 100 mV

Synchronisation Channel

 

Principle

Constant Fraction Discriminator (CFD)

Discriminator Input Bandwidth

4 GHz

Optimum Input Voltage Range

-30 mV to -500 mV

Min. Input Pulse Width

200 ps

Threshold

0 to -250 mV

Frequency Range

0 to 150 MHz

Frequency Divider

1, 2, 4

Zero Cross Adjust

-100 mV to 100 mV

Time-to-Amplitude Converters / ADCs

 

Principle

Ramp Generator / Biased Amplifier

TAC Range

50 ns to 5 µs

Biased Amplifier Gain

1 to 15

Biased Amplifier Offset

0 % to 50 % of TAC Range

Time Range incl. Biased Amplifier

3.3 ns to 5 µs

Min. Time Channel Width

813 fs

ADC Principle

50 ns Flash ADC with Error Correction

Diff. Nonlinerarity

< 0.5 % RMS, typ. < 1 % peak-peak

Data Acquisition

Histogram Mode

Method

on-board multi-dim. histogramming process

Dead Time

80 ns, independent of computer speed

Saturated Count Rate

12.5 MHz

Useful Count Rate

6.25 MHz

Max. Counts / Time Channel

16 bits

Overflow Control

none, stop, repeat and correct

Collection Time

0.1 µs to 100,000 s

Diplay Interval Time

0.1 µs to 100,000 s

Repeat Time

0.1 µs to 100,000 s

Sequencing Recording

Programmable Hardware Sequencer, unlimited recording by Memory swapping, in curve mode and scan mode

Synchronisation with Scanning

Pixel, Line and Frame from Scanning Device

Routing

1 bit, TTL

Experiment Trigger

TTL

Data Acquisition

FIFO / Parameter-Tag Mode

Method

Time and wavelength tagging of individual photons and continuous writing to disk

Online Display

Decay functions, FCS, Cross-FCS, PCH MCS Traces

FCS Calculation

Multi-tau algorithm, online calculation and online fit

Number of counts of decay/ waveform recording

unlimited

Dead Time

80 ns

Saturated Count Rate, Peak

12.5 MHz

Sustained Count Rate (bus transfer limit)

typ. 4 MHz

Output Data Format (ADC / Macrotime / Routing)

12 / 12 / 4

FIFO Buffer Capacity (Photons)

2 * 106

Macro Timer Resolution, Internal Clock

50 ns, 12 bit, overflows marked by MOTF entry in data stream

Input Macro Timer Resolution, Clock from Sync

10 ns to 100 ns, 12 bit, overflow marked by MOTF entry in data stream

Input Curve Control (external Routing)

4 bit, TTL

External Event Markers

4 bit, TTL

Count Enable Control

1 bit, TTL

Input Experiment Trigger

TTL

Data Acquisition

FIFO / Parameter-Tag Imaging Mode

Method

Buildup images from time- and wavelength tagged data

Online Display

up to 8 Images in different time and wavelength windows

Synchronisation with Scanner

via Frame Clock, Line Clock and Pixel Clock Pulses

Detector / Wavelength Channels

1 to 16

Image Resolution (64-bit SPCM Software)

 

No. of Time Channels

64

256

1024

4096

4096

4096

No. of Pixels, 1 Detector Channel

4096 x 4096

2048 x 2048

1024 x 1024

512 x 512

256 x 256

128 x 128

No. of Pixels, 16 Detector Channels

1024 x 1024

512 x 512

256 x 256

128 x 128

128 x 128

128 x 128

Operation Environment

 

PC System

Windows 7 to 10, > 8 GB RAM, 64 bit operating system recommended

PC Interface

PCI / PCIe

Power Consumption

approx. 12 W from +5 V, 0.7 W from +12 V

Dimensions

312 mm x 130 mm x 15 mm

 

The bh TCSPC Handbook
8th edition, corrected and updated September 2019

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