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Wide-Field TCSPC FLIM with bh SPC-150N TCSPC System and Photek FGN 392-1000 Detector

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We present a wide-field TCSPC FLIM system consisting of a position-sensitive MCP PMT of the delay-line type, three SPC-150N TCSPC modules, a bh BDS-SM picosecond diode laser, an inverted microscope, and optics that projects a fluorescence image on the active area of the detector. The operation of the system is fully integrated in the bh SPCM TCSPC software, data analysis is performed by bh SPCImage. The system is able to record FLIM data with 1024 time channels and up to 1024 x 1024 pixels. The effective spatial resolution of the detector / TCSPC combination is about 250 x 250 pixels fwhm, corresponding to about 160 μm on the active area of the detector.

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