The bh FLIM systems for the Zeiss LSM 710 and LSM 710 NLO microscopes [5] are able to record Z stacks of FLIM images. The LSM 710 subsequently scans a number of Z planes. Synchronisation of the FLIM recording with the Z scan is obtained via a z-plane trigger from the microscope. The FLIM system starts a FLIM acquisition for each plane trigger, and repeats the acquisition for the desired number of Z planes. After each plane the data are saved into a file. The result is a sequence of FLIM data files for consecutive planes of the sample.
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