The bh DCS-120 confocal FLIM system in the Zeiss Axio Observer Z1 version is able to record Z stacks of FLIM images. The sample is scanned at a rate on the order of 1 to 10 frames per second. The TCSPC system runs a series of measurement cycles over specified periods of time while actively controlling the Z position of the Axio Observer. After each measurement cycle the data are saved into a file. The result is a number of FLIM data files for consecutive planes of the sample.
