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Recording Z Scans with the DCS-120 Confocal Scanning FLIM System

The bh DCS-120 confocal FLIM system in the Zeiss Axio Observer Z1 version is able to record Z stacks of FLIM images. The sample is scanned at a rate on the order of 1 to 10 frames per second. The TCSPC system runs a series of measurement cycles over specified periods of time while actively controlling the Z position of the Axio Observer. After each measurement cycle the data are saved into a file. The result is a number of FLIM data files for consecutive planes of the sample.

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