The bh FLIM systems for the Zeiss LSM 710 /780 / 880 / 980 family laser scanning microscopes are based on bh’s Multi-Dimensional TCSPC technique and 64 bit Megapixel technology. The systems feature single-photon sensitivity, excellent spatial and temporal resolution, multi-exponential decay analysis, and short acquisition time. The systems are available both for confocal and for multiphoton versions of the Zeiss LSMs. The recording functions include basic FLIM recording, dual-channel FLIM, excitation-wavelength-multiplexed FLIM, Z stack FLIM, time-series FLIM, spatial and temporal mosaic FLIM, fluorescence lifetime-transient scanning (FLITS), and phosphorescence lifetime imaging (PLIM). Multi-spectral FLIM is available by adding a special detector to the system. Unlike other systems, the bh FLIM systems are true molecular imaging systems. They use the fluorescence lifetime not only as a contrast parameter but as an indicator of the molecular state of the sample. This becomes possible by recording the complex multi-exponential decay behaviour in the individual pixels and analysing the data with advanced data analysis procedures based on a combination of MLE-based time-domain analysis, phasor analysis, and image segmentation functions. This brochure gives an overview of the recording functions of the systems, the data analysis, and the application to FRET measurements, autofluorescence FLIM of tissue, metabolic imaging, time-resolved recording of fast physiological processes, and plant physiology. For complete information please see handbook of the bh FLIM systems for the Zeiss LSM series microscopes and addendum for LSM 980 microscopes.
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