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Multiphoton FLIM with the Leica HyD RLD Detectors

Leica have recently introduced hybrid detectors for the non-descanned (RLD) ports of their SP5 and SP8 multiphoton laser scanning microscopes. We have tested these detectors with the bh TCSPC FLIM systems. We describe the TCSPC parameter setup and operating conditions for the detectors, and demonstrate the performance for typical samples.

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