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FLIM Systems for Laser Scanning Microscopes

The FLIM systems are based on bh’s multi-dimensional time-correlated single photon counting (TCSPC) process in combination with confocal or multiphoton scanning by a high-frequency pulsed laser beam. Each photon is characterised by its time in the laser pulse period and the coordinates of the laser spot in the scanning area in the moment of its detection. The recording process builds up a photon distribution over these parameters. The result is an array of pixels, each containing a full fluorescence decay curve in a large number of time channels.

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