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Two-Photon Fluorescence Excitation by Picosecond Diode Lasers

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Picosecond diode lasers can be used for two-photon excitation of typical fluorophores used in biomedical spectroscopy. A 10 mW, 785 nm, 300 ps pulse width, 50 MHz repetition rate laser was focused into a dye cell by an NA = 0.4 microscope lens. The fluorescence was detected by a cooled GaAs-cathode PMT and recorded by time-correlated single photon counting. A count rate of 4500 photons per second was obtained from a 10-3 mol/l Rhodamine 6G solution.

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