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Fluorescence Lifetime Imaging Ophthalmoscopy

Principles, Challenges, Solutions, and Applications

Lecture on Fluorescence-Lifetime Imaging Ophthalmoscopy (FLIO) held April 23, 2021 by Wolfgang Becker. The lecture explains what fluorescence decay functions tell us, how FLIO data are recorded, the way from the raw data to the images, the challenges of FLIO data analysis, solutions to long-existing problems, and new analysis approaches. The last section is on the analysis of FLIO data with SPCImage NG.

Keywords: FLIO, Ophthalmic FLIM, Decay Functions, Multiexponential Decay Analysis

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