Abstract: Abnormally long lifetimes in FLIO (Fluorescence-Lifetime Imaging Ophthalmoscopy) data almost always result from contamination of the fundus fluorescence by fluorescence of the crystalline lens. Here, we show how the problem can be identified and the correct fundus lifetimes be obtained.
Keywords: FLIM, FLIO, Lens Fluorescence, Fundus Fluorescence, Cataract