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Multiphoton Multiwavelength NDD FLIM with Zeiss LSM 710 NLO

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The bh multi-wavelength FLIM systems use multi-dimensional TCSPC to detect FLIM simultaneously in 16 wavelength channels. The fluorescence light is split spectrally by a grating polychromator, the spectrum is projected on a 16-channel PMT, and for each photon a timing signal and a channel-identification signal is generated. The TCSPC module builds up a photon distribution over the time in the signal period, the wavelength and the location of origin of the photons in the scan area. The result is 16 FLIM data sets for the 16 wavelength intervals detected by the 16-channel PMT.

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