The combination of the Abberior STED system with the bh SPC-150 or SPC-160 TCSPC FLIM modules
records FLIM data at a spatial resolution of better than 40 nm. The image format can be as large as
2048 x 2048 pixels with 256 time channels per pixel. Since July 2016 bh SPCM data acquisition
software has an online display function for fluorescence lifetime images. The function can be used to
display STED FLIM images during the acquisition of the data.
Keywords: FLIM, STED