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DCS-120 Confocal Scanning System: FLIM with NIR Dyes

We used the DCS-120 confocal FLIM system to test two typical nearinfrared dyes, indocyanine green (ICG) and methylene blue, as markers in FLIM experiments. Using a 640 nm and 785 nm ps diode laser and near-infrared sensitive hybrid detectors, we were able to detect bright FLIM images from both dyes. Both dyes showed clearly detectable changes in their fluorescence decay parameters depending on the tissue structures they were bound to.

Keywords: FLIM, NIR FLIM, NIR Dyes

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