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DCS-120 Confocal Scanning System: FLIM with NIR Dyes

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We used the DCS-120 confocal scanning FLIM system to test two typical nearinfrared dyes, indocyanine green (ICG) and methylene blue, as markers in FLIM experiments. Using a 640 nm ps diode laser and near-infrared sensitive detectors, we were able to detect bright FLIM images from both dyes. Both dyes showed clearly detectable changes in their fluorescence decay parameters depending on the tissue structures they were bound to.

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