On 457 pages, the 9th edition of the DCS handbook describes the electronical and optical principles of the DCS-120 confocal and multiphoton FLIM systems, basic and advanced techniques and procedures, and system configurations for different applications. A large chapter is dedicated to FLIM applications in biology. It covers the measurement of local environment parameters, protein interaction experiments by FRET, autofluorescence FLIM, metabolic FLIM, and metabolic FLIM in combination with pO2 measurement. Analysis of FLIM data is described in a special chapter on bh’s new SPCImage NG data analysis software. The book ends with a list of 600 references of publications related to FLIM, most of them being applications of the bh TCSPC devices.
Keywords: FLIM, PLIM, TCSPC, Confocal Microscope, Multiphoton Microscope, Molecular Imaging, FRET, Metabolic Imaging