• FLIM of Macroscopic Objects
  • Scan Field Up to 15 mm Diameter
  • Two Fully Parallel TCSPC FLIM Channels
  • Recording by bh's Multidimensional TCSPC Process
  • Compact bh Simple-Tau or Power-Tau TCSPC System
  • Scanning by Fast Galvanometer Mirrors
  • Channel Seperation by Dichroic or Polarising Beamsplitters
  • Individually Selectable Pinholes and Filters
  • One or Two BDL-SMN or BDS-SM ps Diode Lasers
  • Tuneable Excitation by Super-Continuum Laser with AOTF
  • Two Fully  Parallel Confocal Detection Channels
  • Two HPM-100-40 GaAsP Hybrid Detectors
  • Optional: Two HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM
  • Optional: HPM-100-50 Hybrid Detectors for NIR FLIM
  • Optional: Multi-Wavelength FLIM
  • Excellent Time Resolution: Electrical IRF Width 3.5 ps FWHM
  • Time Channel Width Down to 405 fs
  • Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels
  • Simultaneous FLIM/PLIM
  • Wideband Version, Compatible with Tuneable Lasers
  • Electronic Pinhole Alignment
  • Optional: Spatial Mosaic FLIM via Motorized Sample Stage

The DCS-120 MACRO version of the DCS system scans objects directly in the focal plane of the scanner. Objects up to a size of 15 mm can be imaged at a resolution of about 25 micrometers. As the DCS-120 confocal FLIM system, the DCS-120 MACRO uses excitation by ps diode lasers, fast scanning by galvanometer mirrors, confocal detection, and FLIM by bh’s multidimensional TCSPC technique to record fluorescence lifetime images at high temporal resolution, high spatial resolution, and high sensitivity. Due to its fast beam scanning and its high sensitivity the DCS-120 MACRO system is compatible with live-object imaging. DCS-120 functions include simultaneous recording of FLIM or steady-state fluorescence images simultaneously in two fully parallel wavelength channels, laser wavelength multiplexing, time-series FLIM, time-series recording, Z stack FLIM, phosphorescence lifetime imaging (PLIM), fluorescence lifetime-transient scanning (FLITS) and FCS recording. Applications focus on lifetime variations by interactions of fluorophores with their molecular environment. Typical applications are ion concentration measurement, FRET experiments, metabolic imaging, imaging of fast physiological effects, and plant physiology.

Please see

DCS-120 Confocal and Multiphoton FLIM Systems, User Handbook, 8th Edition, pages 381-383

The realm of the bh FLIM systems are in molecular imaging. Typical applications are the imaging of ion concentrations, pH, or local viscosity, protein interaction experiments by FRET, and metabolic imaging by fluorescence decay of NADH an FAD in combination with oxygen measurement. In these applications, the bh FLIM systems benefit from their high sensitivity, high time resolution, high timing stability, and their capability to resolve multi-exponential-decay profiles into their components. Other advantages are the capability to record FLIM of fast physiological effects down to the millisecond range, and to record at several excitation and emission wavelengths simultaneously.

The bh TCSPC Handbook
8th edition, corrected and updated September 2019


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