DPC-230 TCSPC Module

Discontinued - Please Use SPC-QC-004 or SPC-QC-104

  • Time Channel Width Down to 165 ps
  • 16 LVTTL Inputs for SPADs or 4 CFD Inputs for PMTs
  • Recording of Absolute Photon Times
  • Multiscaler Operation (Single-Start, Multi-Stop)
  • Free Instrument Software for Windows 8/10
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Description

The DPC-230 is a photon correlator module that records absolute photon times in up to 16 parallel detection channels. The board can be used for traditional fluorescence lifetime measurements, single molecule spectroscopy, FCS and FCCS recording, FLIM. The board offers a PCI interface and can be easily installed in nearly all PCs.

General Information

The DPC-230 especially targets at a new type of fluorescence correlation spectroscopy that records correlation over a time range of 10 orders of magnitude. Thus, singlet decay rates, rotational relaxation rates, rate constants of conformational changes, triplet transition and decay rates, and diffusion times of free fluorophores and fluorophores bound to proteins, lipids or nanoparticles can be obtained within a single measurement. Another application may be diffuse optical correlation experiments which require autocorrelation in a large number of detection channels.
The DPC-230 photon correlator card records absolute photon times in up to 16 parallel detection channels. The time resolution of the recording is 165 ps per time channel. Depending on how the photons are correlated, fluorescence correlation (FCS), fluorescence cross correlation (FCCS), or photon counting histograms are obtained. Moreover, the DPC-230 can be used for recording luminescence decay curves and fluorescence lifetime images from the sub-nanosecond to the millisecond range, for LIDAR experiments, time-of flight mass spectroscopy, or any other experiments based on acquiring the temporal distribution of detection events in a large number of detector channels.

Measurement Software Included

The SPCM operating and measurement software is included with all SPC series modules. SPCM provides online calculation and display (2D, 3D) of data (decay curves, FLIM, FCCS) acquired in multiple operation modes. SPCM software undergoes active continuous development. SPCM receives frequent updates with new features and bug fixes. Read more…

FLIM and FCS Data Analysis

For advanced fluorescence lifetime imaging (FLIM) and single-curve data analysis, please use SPCImage.
For advanced fluorescence correlation spectroscopy (FCS) and cross-correlation (FCCS) data analysis, please use Burst Analyzer.

Custom Programming Libraries (DLL, LabVIEW)

For automation and custom software integration, DLLs and LabVIEW drivers are available. Read more…

Specifications

LVTTL Input

 

No. of Channels

16

Input Voltage

LVTTL

Threshold

1.4 V

Min. Input Pulse Width

2 ns

Min. Pulse Distance

5.5 ns

Connectors

MCX, on board

CFD Input

 

No. of Channels

4

Threshold

-20 mV to -500 mV

Zero Cross Adjust

-100 mV to 100 mV

Connectors

SMA, front panel

Experiment Trigger Input

 

Input Voltage

LVTTL

Threshold

1.4 V

Data Acquisition

Absolute Time Mode

Method

Time-Tag recording, absolute photon times

Correlation of Photons

Multi tau or Linear tau algorithm, online or offline

Autocorrelation

all channels

Cross-Correlation

any pairs of channels

Time Increment

164.61

Dead Time

< 10 ns

No. of Parallel Channels

16 LVTTL or 4 CFD channels

On board FIFO Buffer Size

4 * 106 photons

Readout

continuous readout during measurement

Sustained Readout Rate

7 * 106 photons

Online Calculation

FCS, FCS Fit, MCS, PCH

Data Acquisition

TCSPC Mode

Method

Time-Tag recording, reversed start-stop

Correlation of photons

Start-stop histogram, online or offline

Start (Photon) Channels

3 CFD inputs

Dead Time

< 10 ns

Stop Channel

1 CFD input

Stop Input Rate

max. 150 MHz

Stop Frequency Divider

1, 2, 4

Time Channel Width

164.61 ps

On board FIFO Buffer Size

4 * 106 photons

Readout

continuous readout during measurement

Sustained Readout Rate

7 * 106 photons

Online Calculation

Deacy, FCS, FCS Fit, MCS, PCH, Image

Data Acquisition

Multiscaler Mode

Method

Time-Tag recording, direct start-multistop

Correlation of Photons

Start-stop histogram, online or offline

Start (Reference) Channel

1 CFD input or 1 LVTTL input

Stop (Photon) Channels

3 CFD inputs or 15 LVTTL inputs

Dead Time

< 10 ns

Time Channel Width

164.61 ps

On board FIFO Buffer Size

4 * 106 photons

Readout

continuous readout during measurement

Sustained Readout Rate

7 * 106 photons

Online Calculation

Deacy, FCS, FCS Fit, Antibunching, MCS, PCH, Image

Imaging Operation

Available for

TCSPC and Multiscaler Mode

Acquisition Principle

Scanning, recording of Pixel, Line, and Frame Clock Pulses

Max. Pixel Rate

2 MHz

Operation Environment

 

PC System

Windows 8 / 10, > 8 GB RAM, 64 bit operating system recommended

PC Interface

PCI

Power Consumption

approx. 12 W from +5 V, 0.7 W from +12 V

Dimensions

312 mm x 130 mm x 15 mm

Downloads

Documents

The bh TCSPC Handbook
10th edition, September 2023

View

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