Starting from software version 9.76, bh SPCM data acquisition software controls a motorised sample stage. In combination with the motorised stage, the DCS-120 confocal or multiphoton FLIM systems record mosaics of FLIM images. The system scans an image at one position of the sample, then offsets the sample by the size of the scan area, and scans a new image. The process is repeated, combining the data of the individual scans into a single, large x-y-t data set. Images covering an area of several mm diameter can be obtained without the need of using low-magnification and low-NA objective lenses.
Keywords: FLIM, Mosaic FLIM, Motor Stage