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Two-Photon FLIM of Pollen Grains Reveals Ultra-Fast Decay Component

Using our DCS-120 MP multiphoton FLIM system with ultra-fast hybrid detectors, we find extremely fast fluorescence-decay components in a wide variety of biological material. Here, we report on FLIM of pollen grains from a number of different plants. We found decay components with lifetimes, t1, from 10 ps to 80 ps, and with amplitudes,  a1, as large as 93 %.

Keywords: FLIM, Ultra-Fast Fluorescence Decay, Pollen, Multiesponential Decay Analysis

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