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DCS-120 Confocal Scanning FLIM System: Two-Photon Excitation with Non-Descanned Detection

This application note shows how the bh DCS-120 confocal FLIM system can be upgraded for multiphoton FLIM with non-descanned detectors. With the upgraded system, highly detailed lifetime images from unstained mammalian skin could be recorded from as deep as 90 μm below the surface. The efficiency of the system is excellent. An average count rate of 1 MHz could be obtained without detectable photobleaching within an acquisition time of two minutes. The quality of the data obtained within this time was adequate for triple-exponential decay analysis.

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