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An AFM/NSOM System with Fluorescence Lifetime Imaging

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We present the integration of fluorescence lifetime imaging (FLIM) into an atomic force microscope (AFM). The system is based on the NTEGRA SPECTRA AFM/NSOM system of NT-MDT, Moscow, and a Simple-Tau 150 TCSPC FLIM system of Becker & Hickl, Berlin. We demonstrate that the integrated system is able to record combined AFM / FLIM images of E-coli bacteria, HEK cells, and polymers.

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