The DCS-120 MP multiphoton FLIM system is an extended version of the bh DCS-120 confocal scanning FLIM System. It uses multiphoton excitation by a femtosecond titanium-sapphire laser, fast galvanometer scanning, non-descanned detection, hybrid detector technology, and single-photon recording by bh’s multi-dimensional TCSPC process. An AOM is included to control the laser power and to modulate the laser for PLIM acquisition. The system records FLIM data in two fully parallel recording channels, runs Z stacks, accumulates fast FLIM time series, and records simultaneously FLIM and PLIM.
Keywords: FLIM, PLIM, Multiphoton