- Control All bh’s TCSPC-Hardware and Experiment Parameters with NIS Elements Software
- 4x bh Picosecond Diode Lasers, 60 ps to 120 ps Pulse Width (FWHM (1)) with Three Repetition Rates: 20 MHz, 50 MHz, 80 MHz and CW
- Up to 3x Parallel Detection Channels Equipped with B&H HPM Detectors Featuring an IRF ~ 20 ps FWHM (2)
- High Photon Count Rates Up to 80 MHz Continuously and 120 MHz Peak Count, 4 ps Time-Channels, Time Jitter ~11 ps (3)
- Motorized Sample Movement for Imaging, 3D-FLIM
- Seamlessly Switchable Emission Filters
(1) Wavelength Based; (2) Detector Based; (3) Divided by /√2
Description
Record Fluorescence Lifetime Decay in Every Individual Pixel Ensuring no Detail is Missed
With its three input channels, picoseconds time precision and an extremely high-count rates detection, the SPC-QC-104 module records phenomenal FLIM images with an instant 1st-moment fit for a quick contrast-FLIM analysis or further multi-exponential fit analysis within a matter of seconds. Featuring near-ideal photon efficiency and minimal acquisition time, the B&H’s acquired FLIM images exhibit pixel-level precision, yielding detailed fluorescence decay profiles across a numerous number of picosecond-accurate time channels.
Once recorded, the images can be swiftly exported to B&H’s renowned SPCImage software, opening the door for a rapid precise fitting, image segmentation, phasor plot analysis and more, facilitating advanced analysis and interpretation of the data. Calculated in every pixel, SPCImage creates colored-coded images of the amplitude- or intensity-weighted lifetimes, amplitude of the decay components, amplitude ratios, or other combinations of the decay parameter, such as FRET intensities, distances and fraction of interacting proteins, bound and unbound fluorophores or the fluorescence lifetime redox ration FLIRR.
Specifications
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Selected Specifications |
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Principle |
TCSPC FLIM by bh´s Multi-dimensional TCSPC technique, fully integrated in Nikon NIS |
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Lifetime Measurement |
Time-domain single-photon counting |
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Excitation |
ps pulsed lasers, fiber coupled |
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Scan Rate |
Supports full Nikon AX scan rate range |
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General Operation Modes |
TCSPC FLIM:
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TCSPC System |
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TCSPC / FLIM Modules |
SPC-QC-104 |
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Number of Parallel TCSPC / FLIM Channels |
Typ. 2 |
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Electrical Time Resolution |
16 ps RMS / <39 ps FWHM |
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Timing Precision / |
11 ps |
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Minimum Time Channel Width |
4 ps |
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Saturated Count Rate |
40 MHz, shared among active channels. |
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Software |
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Data Acquisition Software |
NIS Elements software |
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Operation System |
Windows 10 / 11 64 bit |
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Data Analysis Software |
bh SPCImage NG |
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Principle of Data Analysis |
MLE with GPU processing |
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Model of Functions |
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IRF Modelling |
Syntethic IRF function fit to decay data, Auto-extraction of IRF from data, or measured IRF |
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Excitation Sources |
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For Confocal FLIM |
1 - 4 bh ps pulsed diode lasers |
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Available Wavelength |
405 nm to 785 nm |
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Repetition Rate |
20, 50, 80 MHz and CW |
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Pulse Width |
40 ps to 100 ps |
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Detectors |
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Confocal Detectors |
Coupled directly to Nikon AX confocal port |
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Standard Detector |
HPM-100-40 hybrid detector with GaAsP cathode, 250 to 720 nm, best for use with ns lifetime dyes |
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Optional |
HPM-100-06 detector with <20 ps FWHM IRF width, 220 to 650 nm, best for ps autofluorescence lifetime samples |
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Optional |
HPM-100-50 detector, 400 to 900 nm, best for long wavelength fluorescence |
Downloads
Documents
Datasheets
Gallery
