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Mosaic FLIM: New Dimensions in Fluorescence Lifetime Imaging

Mosaic FLIM acquires several (usually a large number of) TCSPC-FLIM recordings into a single, large FLIM data set. The data set can represent a spatial mosaic of images, a Z stack of images, a time series of images, or images recorded in different wavelength intervals of a multi-wavelength detector. The advantage of the technique is that no time for saving the individual images needs to be reserved, and that extremely fast time series can be recorded by triggered accumulation. We demonstrate the technique for a bh TCSPC FLIM system attached to a Zeiss LSM 710 NLO and a Zeiss LSM 7 NLO microscope.

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