- Fast PCIe Interface
- Minimum Time Channel Width 407 fs
- Ultra-High Time Resolution 3.5 ps FWHM, Internal Timing Jitter 1.5 ps RMS
- Excellent Timing Stability
- Ideal for Ultra-Fast HPM (Hybrid) Detectors and Superconducting NbN Detectors
- 12 MHz Saturated Count Rate
- Precision Fluorescence Decay Recording
- High-Resolution FLIM
- Simultaneous FLIM/PLIM
- Multi-Wavelength FLIM
- Realtime FLIM and FCS Recording
- Photon Correlation
- Single-Molecule Spectroscopy
- Free Instrument Software for Windows 7/8/10 (Realtime Calculation and Fitting of FCS Curves)
- Link to SPCImage NG Data Analysis
- Parallel Operation of Up to 4 Modules
- Available as Multi-Module Package e.g. SPC-182NX, SPC-183NX and SPC-184NX
|
Photon Channel |
|
|||
|
Principle |
Constant Fraction Discriminator (CFD) |
|||
|
Discriminator Input Bandwidth |
4 GHz |
|||
|
Time Resolution (FWHM/RMS, electr.) |
< 3.5 ps / 1.6 ps |
|||
|
Variance in Time of IRF centroid |
< 0.4 ps RMS over 100 s |
|||
|
Optimum Input Voltage Range |
-30 mV to -500 mV |
|||
|
Min. Input Pulse Width |
200 ps |
|||
|
Threshold |
0 to -250 mV |
|||
|
Zero Cross Adjust |
-100 mV to 100 mV |
|||
|
Synchronisation Channel |
|
|||
|
Principle |
Constant Fraction Discriminator (CFD) |
|||
|
Discriminator Input Bandwidth |
4 GHz |
|||
|
Optimum Input Voltage Range |
-30 mV to -500 mV |
|||
|
Min. Input Pulse Width |
200 ps |
|||
|
Threshold |
0 to -250 mV |
|||
|
Frequency Range |
0 to 150 MHz |
|||
|
Frequency Divider |
1, 2, 4 |
|||
|
Zero Cross Adjust |
-100 mV to 100 mV |
|||
|
Time-to-Amplitude Converters / ADCs |
|
|||
|
Principle |
Ramp Generator / Biased Amplifier |
|||
|
TAC Range |
25 ns to 2.5 µs |
|||
|
Biased Amplifier Gain |
1 to 15 |
|||
|
Biased Amplifier Offset |
0 % to 50 % of TAC Range |
|||
|
Time Range incl. Biased Amplifier |
1.67 ns to 2.5 µs |
|||
|
Min. Time Channel Width |
407 fs |
|||
|
ADC Principle |
50 ns Flash ADC with Error Correction |
|||
|
Diff. Nonlinerarity |
< 0.5 % RMS, typ. < 1 % peak-peak |
|||
|
Data Acquisition |
Histogram Mode |
|||
|
Method |
on-board multi-dim. histogramming process |
|||
|
Dead Time |
80 ns, independent of computer speed |
|||
|
Saturated Count Rate |
12 MHz |
|||
|
Max. Counts / Time Channel |
16 bits |
|||
|
Overflow Control |
none, stop, repeat and correct |
|||
|
Collection Time |
0.1 µs to 100,000 s |
|||
|
Diplay Interval Time |
10 ms to 100,000 s |
|||
|
Repeat Time |
0.1 µs to 100,000 s |
|||
|
Sequencing Recording |
Programmable Hardware Sequencer, unlimited recording by Memory swapping, in curve mode and scan mode |
|||
|
Synchronisation with Scanning |
Pixel, Line and Frame from Scanning Device |
|||
|
Routing |
7 bit, TTL |
|||
|
Experiment Trigger |
TTL |
|||
|
Data Acquisition |
FIFO / Parameter-Tag Mode |
|||
|
Method |
Time and wavelength tagging of individual photons and continuous writing to disk |
|||
|
Online Display |
Decay functions, FCS, Cross-FCS, PCH MCS Traces |
|||
|
FCS Calculation |
Multi-tau algorithm, online calculation and online fit |
|||
|
Number of counts of decay/ waveform recording |
unlimited |
|||
|
Dead Time |
80 ns |
|||
|
Saturated Count Rate, Peak |
12 MHz |
|||
|
Sustained Count Rate (bus transfer limit) |
typ. 5 MHz |
|||
|
Max. Counts / Time Channel (Counting Depth) |
unlimited |
|||
|
Output Data Format (ADC / Macrotime / Routing) |
12 / 12 / 4 |
|||
|
FIFO Buffer Capacity (Photons) |
2 * 106 |
|||
|
Macro Timer Resolution, Internal Clock |
25 ns, 12 bit, overflows marked by MOTF entry in data stream |
|||
|
Input Macro Timer Resolution, Clock from Sync |
10 ns to 100 ns, 12 bit, overflow marked by MOTF entry in data stream |
|||
|
Input Curve Control (external Routing) |
4 bit, TTL |
|||
|
External Event Markers |
4 bit, TTL |
|||
|
Enable Count Control |
1 bit, TTL |
|||
|
Input Experiment Trigger |
TTL |
|||
|
Data Acquisition |
FIFO / Parameter-Tag Imaging Mode |
|||
|
Method |
Buildingup images from time- and wavelength tagged data |
|||
|
Online Display |
up to 8 Images in different time and wavelength windows |
|||
|
Synchronisation with Scanner |
via Frame Clock, Line Clock and Pixel Clock Pulses |
|||
|
Detector / Wavelength Channels |
1 to 16 |
|||
|
Image resolution (64-bit SPCM Software) |
|
|||
|
No. of Time Channels |
64 |
256 |
1024 |
4096 |
|
No. of Pixels, 1 Detector Channel |
4096 x 4096 |
2048 x 2048 |
1024 x 1024 |
512 x 512 |
|
No. of Pixels, 16 Detector Channels |
1024 x 1024 |
512 x 512 |
256 x 256 |
128 x 128 |
|
Operation Environment |
|
|||
|
PC System |
Windows 7 to 10, > 8 GB RAM, 64 bit operating system recommended |
|||
|
PC Interface |
PCIe |
|||
|
Power Consumption |
approx. 12 W from +12 V |
|||
|
Dimensions |
230 mm x 130 mm x 18 mm |
|||
Applications
Application Notes
- Two-Photon Fluorescence Excitation by Picosecond Diode Lasers
- Time-Tag Recording: A New Old Feature of the bh SPC Cards
- FLIM in the FIFO Imaging Mode: Large Images with Small TCSPC Modules
- Recording the Instrument Response Function of a Multiphoton FLIM System
- Recording Z Scans with the DCS-120 Confocal Scanning FLIM System
- Tuneable Excitation FLIM with the LSM 710 Intune System
- An 8-Channel Parallel Multispectral TCSPC FLIM System
- Microsecond Decay FLIM: Combined Fluorescence and Phosphorescence Lifetime Imaging
- Spatially Resolved Recording of Fluorescence-Lifetime Transients by Line- Scanning TCSPC
- Combined Fluorescence and Phosphorescence Lifetime Imaging (FLIM / PLIM) with the Zeiss LSM 710 NLO Microscopes
- TCSPC at Wavelengths from 900 nm to 1700 nm
- FLIM and FCS by Pulse-Interleaved Excitation with the Zeiss LSM 710/780 Intune System
- Mosaic FLIM: New Dimensions in Fluorescence Lifetime Imaging
- Zeiss BiG-2 GaAsP Detector is Compatible with bh FLIM Systems
- An AFM/NSOM System with Fluorescence Lifetime Imaging
- NSOM FLIM with the Nanonics AFM/NSOM System
- Implantable Fibre-Optical Fluorescence-Lifetime Detection System for in-vivo Applications
- TCSPC Fibre-Probe System with an Exchangeable Tip
- bh FLIM Systems Record Calcium Transients in Live Neurons
- The PZ-FLIM-110 Piezo-Scanning FLIM System
- 80 ps FHWM Instrument Response with ID230 InGaAs SPAD and SPC-150 TCSPC Module
- World Record in TCSPC Time Resolution: Combination of bh SPC-150NX with SCONTEL NbN Detector yields 17.8 ps FWHM
- bh – Abberior Combination Records STED FLIM at Megapixel Resolution
- SPCM Software Runs Online-FLIM at 10 Images per Second
- bh TCSPC Systems Record FLIM with Sutter MOM Microscopes
- Sub-20ps IRF Width from Hybrid Detectors and MCP-PMTs
- Ultra-fast HPM Detectors Improve NAD(P)H FLIM
- TCSPC System Records FLIM of a Rotating Object
- 4.4 ps IRF width of TCSPC with an NbN Superconducting Nanowire Single Photon Detector
- Influence of Magnetic Fields on the IRF of High-Speed Detectors for TCSPC
- Two-Photon FLIM of Mushroom Spores Reveals Ultra-Fast Decay Component